Abstract:

To establish a method for identification of carotenoids in chinese cabbage, reversed phase high-performance liquid chromatography (RP-HPLC), coupled with carotenoid standards and photodiode array detection was used to analyze caotenoid composition in different genotypes of chinese cabbage. 48 kinds of carotenoids were separated on a C30 column, and 30 of them were identified on the basis of retention time and spectral characteristics with standards. Among which neurosporene, zeaxanthin, phytofluene, ζ-carotene, γ-carotene and violaxanthinand were first identified from chinese cabbage. The kinds of carotenoid varied among genotypes and those in orange-heading chinese cabbage were the most. The results also indicated that this method was suitable for identification of carotenoids in chinese cabbage.