Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2011, Vol. 27 ›› Issue (19): 100-104.

Special Issue: 生物技术 园艺

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Clone and Expression Profile of A Cold-inducible Gene of Camellia sinensis

  

  • Received:2011-01-20 Revised:2011-03-07 Online:2011-08-05 Published:2011-08-05

Abstract:

Study about the mechanism of cold acclimation of tea plants is of importance for elucidating the frost resistance of perennial plants and breeding cold-resistance variety of tea plants. In present paper, CsCOR1 gene was cloned from tea leaves by RACE approach, and the expression profile of CsCOR1 gene were analyzed by Real-time RT PCR strategy under cold, hyperosmosis and ABA-treatment. The results show that CsCOR1 gene encodes a 86 amino-acid peptide, which consists of a hydrophobic N-terminal region for a signal peptide and a hydrophilic C-terminal domain that is rich in glycine,arginine and proline. There are two internal repeat sequences in mature CsCOR1. CsCOR1 expression was induced dramatically by cold/hyperosmosis and moderately by ABA in tea leaves via real-time RT PCR analysis.

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