Abstract:

In order to obtain white period leaves genes in Anji Bacha, cDNA-AFLP amplification reaction system was established and optimized, and cDNA-AFLP was analyzed. In the experiment of leaf in Anji Bacha, the optimization of cDNA-AFLP reaction system was studied by employing white and green period leaves as samples. Different levels of concentration of primer, dNTP mixture, Mg2+ and Taq DNA polymerase were studied in 20 μL amplification system. The results showed that amplification effects are good when the volume of primer concentration was 75 ng/20 μL, Mg2+ concentration was 2.0 mM, dNTP concentration was 0.2 mM, Taq DNA polymerase was 1 U in 20 μL pre-amplification system and the volume of primer concentration was 75 ng/20 μL, Mg2+ concentration was 2.5 mM, dNTP concentration was 0.2 mM, Taq DNA polymerase was 1.5 U in 20 μL selective amplification system. This test obtained better pre-amplification and selective amplification system.