Abstract:

The polymorphism of RBP4 gene in fat-tailed sheep and small tailed han sheep was studied by PCR-SSCP method to explore the influnce of this gene on fecundity of henan landrance sheep breeds. The results showed that: Two genotypes of RBP4 including BB and AB were detected, while only AA genotype was not detected. Frequency of BB genotype, AB genotype in small tailed han sheep and fat-tailed sheep were 0.840 and 0.587, 0.160 and 0.416 respectively. A allele frequency and B allele frequency in small tailed han sheep and fat-tailed sheep were 0.080 and 0.209, 0.920 and 0.791 respectively. This study provide reference for exploring high fecundity mechism of Henan landrance sheep breeds at molecular level.