Abstract:

Seven normally matured Inner Mongolian White Cashmere Goat’ semens being removed seminal plasma by centrifugation were cryopreservated.The result from the sperms being removed seminal plasma by centrifugation at 750rpm/min once or twice indicates that the rate of plasma membrane integrity and mitochondrial activity is apperently higher than the control group（P＜0.05）. Acrosome intact integrity rates of cryopreservated sperm in 750rpm/min once group is apperently higher than that of 750rpm/min twice group .The rate of the motility，acrosome integrity of the rates, mitochondrial activity of seminal plasma being removed by centrifugation at 1500rpm/min once is obviously lower than that of the other groups（P＜0.05）. There is no signicant difference in the rate of sperm motility after thawing and the control group.The fact that the higher speed of the centrifugation would give rise to mechanical damage can account for all these.