Abstract:

The study aimed to investigate the proper conditions and influential factors in order to best establish a trophoblastic stem-like cell line, derived from in vitro matured and fertilized blastocysts. The cells were cultured on collagen coated wells using fetal bovine fibroblast-conditioned medium with an adjustable culture system used to investigate the effect on cell growth. We found the the DMEM/F12 medium was mixed with fetal bovine fibroblast-conditioned medium at a dilution of 1:1 and supplemented with FBS, Heparin and β-mercaptoethanol was the best culture medium.