Abstract:

Abstract: The real-time fluorescent quantitative PCR technology combines the traditional PCR and spectroscopy’s advantages; it is more sensitive, rapid and specific. It can detect the changes of fluorescencesignal in the process of PCR to obtain quantitative results. It’s molecular biology diagnostic techniques widely used in animal disease detections because of it’s specificity, sensitivity, reproducibility, quantity, rapid and capping. In this paper, real-time fluorescent quantitative PCR technology on diagnosis of swine virus disease was reviewed.