Abstract:

The aim was to obtain the high quality DNA extracted method from leaves of black walnut which contained polysaccharides and polyphenolic compounds. Using leaves of Black walnut and walnut as experimental materials, the genomic DNA extraction of three methods namely SDS, CTAB and modified CTAB were compared. The DNA samples extracted by three methods were tested by agarose gel electrophoresis, ultraviolet spectrophotometer. The results showed that repeatability of the methods of SDS and CTAB were poor, it was difficult to obtain clear bands by agarose gel electrophoresis. Utilizing modified CTAB method, formation of oxidized polyphenolic compounds could be inhibited and most of polysaccharides in the cytoplasmic could be removed efficiently in leaves. A260/A280 was ranged from 1.80-1.95. The modified CTAB method was suitable for extracting high qualified DNA.