Abstract:

In order to research the effect of apple polyphenols of different variety and maturity on angiotensin converting enzyme activity, HPLC method was established for the analysis of ACE inhibitory activity by apple polyphenols. Effect of variety (‘Fuji’ and ‘Ralls’) and maturity on the inhibition of ACE activity was studied in this research. The results showed that, when the concentration of apple polyphenols were at the range of 1~250μg/mL, the inhibition of ACE activity by four groups of unripe Fuji, ripe Fuji, unripe Ralls, ripe Ralls were more and more stronger. And IC50 value of unripe ‘Fuji’ apple polyphenols and ripe ‘Ralls’ apple polyphenols were 16.9, 80.8 μg/mL, respectively. We can get the conclusion that unripe apple polyphenols had the best effect on the inhibition of ACE activity, and can be used as nature sources of ACE inhibitors.