Abstract:

Experiment was conducted with anthers of three lines of Ningxia Chinese jujube, culturing in vitro and gained some regeneration plant, for studying the effects of different phytohormone and their levels in MS medium on the callus induction and adventitious bud regeneration. The results showed that 6-BA acted as one of important factor in induce embryogenic callus of Ningxia Chinese jujube. After orthogonal test analysis, the best induce medium was MS+6-BA 1.0 mg/L+2,4-D 1.0 mg/L+IBA 0.4 mg/L. AgNO3 played a important role in differentiation. The suitable medium was MS+6-BA 1.5 mg/L +IBA 0.6 mg/L + AgNO3 0.5 mg/L. Lingwu Long jujube had the highest inductivity in the best induced medium which was 89%; Tongxin round jujube had the highest differentiation rate in the best differential medium which was 88%.