Abstract:

The aim was to develop a rapid and efficient method of in vitro shoot regeneration for large scale propagation of Pyrus ussuriensis Maxin. Shoot segments from one-year-old shoots were cultured on MS basal medium supplemented with 6-BA, NAA and IBA. The results showed that the best time was the first ten-day period of April. The best pretreatment was GA3 with 0.1 mg/L concentration for one week. The best sterilization time was 8-10 minutes with 0.1% HgCl2. Different cultivars had different ability of shoot proliferation and growth. The best response in terms of frequency of shoot regeneration and the number of shoots per shoot explants was observed on medium supplemented with 4.0 mg/L 6-BA and 0.4 mg/L NAA. IBA facilitated shoot rooting, and the best medium was 1/2MS+IBA 1.4 mg/L. GA3 inhibited shoot rooting. The best substance was pearlite:vermiculite:sand=1:1:1:1 with 93.3% survival rate.