Abstract:

The seeds and stem segments of Shorea wantianshuea were used as explants to study the methods of disinfection and browning prevention, which will be helpful to get sterile explants for tissue culture. The results showed that it was better to use embryos explants than the seeds and adding in penicillin with 6 millions units was efficient to lower down the contamination rate during disinfecting. It was difficult for stem segments to be disinfected. Penicillin could reduce contamination rate to some extend, but the effects were not good and there was seriously browning during disinfection. The vitamin C (VC), polyvinylpyrrolidone (PVP) and activated carbon (Ac) could reduce the browning effectively with and optimal combination 0.2 mg/L VC, 2 g/L polyvinylpyrrolidone (PVP) and 0.5 g/L activated carbon (Ac) in 1/2 MS medium with and optimal combination.