Abstract:

In the study, we are aimed to investigate the antimicrobial activity of isoalantolactone against Staphylococcus aureus, and further assess the influence of isoalantolactone on the expression of enterotoxins by S.aureus. A broth micro-dilution method was used to determine the minimal inhibitory concentrations(MICs); Western blot, proteolytic activity, TNF-α release and real-time RT-PCR assays were performed to assess the effects of isoalantolactone on S.aureus SEA and SEB expression. The data showed that the MICs of isoalantolactone against S.aureus strains were greater than 2048 μg/mL. However, low concentrations of isoalantolactone (1-8 μg/mL) could dose-dependently inhibit the expression of SEA and SEB by S.aureus.