Abstract:

In this study, by adding different concentration of Vascular Endothelial Growth Factor (VEGF) on in-vitro maturation of porcine occytes, will investigate the suitable concentration of VEGF for the porcine oocytes and therefore improve its embryo maturity in the late stage, which would be the reference for the further improvement of the maturity of porcine oocytes. The different concentrations of VEGF were added into the maturation medium (TCM199+BSA), they were treated as following: the Control group: the porcine oocytes were cultured in the maturation medium without VEGF, GroupⅠwith 5 ng/mL VEGF, Group II with 50 ng/mL VEGF, Group III with 500 ng/mL VEGF respectively. Then, these oocytes were treated by parthenogenesis or in vitro fertilization and vitro culture respectively. The results were shown that: (1) the maturation rate of porcine oocytes in GroupⅠwas significantly higher than that of the control one, and the appropriate maturation rate in GroupⅡ was much higher than that of the control one; (2) The cleavage rate and blastocyst rate of their parthenogenetic embryos in Group I were significantly higher than that of the control one, in addition, this cleavage rate in Group II was much higher than that of the control one, as well as blastocyst rate was significantly higher than that of the control one; (3) While in their in vitro fertilization and in vitro culture, The cleavage rate and blastocyst rate of in-vitro fertilized embryos in Group I were all much higher than that of the control one, in addition, The cleavage rate of in-vitro fertilized embryos in Group II was all much higher than that of the control one, and the blastocyst rate was significantly higher than that of the control one. Collectively, it could be concluded that the concentration of 5 ng/mL or 50 ng/mL VEGF be beneficial to both in vitro maturation of porcine oocytes and their appropriate in vitro development.