Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2013, Vol. 29 ›› Issue (5): 17-20.doi: 10.11924/j.issn.1000-6850.2012-2096

Special Issue: 畜牧兽医

• 23 • Previous Articles     Next Articles

The Standardization of the Collection and Treatment Methods for Swine Nasal Swab Sample

  

  • Received:2012-06-04 Revised:2012-06-21 Online:2013-02-15 Published:2013-02-15

Abstract: The comparison and stability of detecting data were determined by the standard of materials and methods applied to collect mucosal samples. This study combined nasal cavity construction in pig and the specificity of the samples to investigate and standardize the collection and treatment methods of nasal swabs. The results showed that the total protein of 116 nasal swabs, collected by the methods standardized in this study, were detected, found that the total protein mean of all nasal swabs was (6.92±0.41) mg/mL and coefficient of variation was 32.58%. Besides, total protein content of the nasal swabs collected in five different times from 7 pigs were detected and the coefficient of variation of each pigs were below 10%, except one was 13.95%. The standard of the collection and treatment methods for nasal swabs made the detected results more stability and scientific.