Abstract:

Clubroot is a serious soilborne disease of cruciferous crops, the loss cause by the disease was above 10% of the total cultured area world-wide. And investigate the best inoculation method of the disease is important to develops the related or similar search. In this test, five kind inoculation methods, different seed pretreatment, different inoculation concentration and soil pH were measured. As the result, sealing the mixed hypnospore soil before inoculation could get higher disease index than not sealing the soil, and dipping seeds in hypnospore suspension before seeding in the sealed mixed hypnospore soil were more effective than other methods for induce pothogenesy. Contrast the result of the test under different inoculation condition, we found that dipping seed in sterile water 1 day before dipping in hypnospore suspension would reduce the inoculation effect, and the concentration of hypnospore was positive correlate with the disease incidence, the acid condition was fit to clubroot break out.