Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2013, Vol. 29 ›› Issue (23): 10-14.doi: 10.11924/j.issn.1000-6850.2013-0930

Special Issue: 生物技术 畜牧兽医 园艺

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The Effects of Procyanidin from Grape Seed on the Splenic Lymphocytes and Hepatocytes Proliferation from Rat

  

  • Received:2013-04-02 Revised:2013-05-06 Online:2013-08-15 Published:2013-08-15

Abstract: To study of the effects of GSP (Procyanidin from Grape Seed) on the Splenic Lymphocytes and hepatocytes proliferation from Rat, investigate the mechanism to improve immune performance of rats, provide a theoretical basis for the follow-up test group. The experiments used a single factor design. The splenic lymphocytes were treated with different concentration of GSP [0.00 (control group), 0.05, 0.10, 0.50, 1.00, 5.00, 10.00, 15.00 μg/mL ] and the hepatocytes were treated with different concentration of GSP [0.00 (control group), 0.05, 0.10, 0.50, 1.00, 5.00, 10.00, 15.00, 20.00, 25.00 μg/mL ]. Procyanidins incubated with these cells to 12 h, 24 h and 48 h, respectively. The plates were read at 490 nm. The results showed that: (1) for the lymphocytes of Splenic, the concentrates of GSP ranging from 0.05 μg/mL to 5.00 μg/mL had promoted effect to the lymphocytes proliferation, while the concentrates of GSP enhanced reach to 10.00 μg/mL, the proliferation of splenic lymphocytes was inhibited; (2) for the hepatocytes IAR-20, the concentrates of GSP ranging from 0.05 μg/mL to 10.00 μg/mL had promoted effect to the hepatocytes proliferation, while the concentrates of GSP enhanced reach to 10.00 μg/mL, the proliferation of hepatocytes was inhibited; (3) Within a certain range, GSP exhibited concentration and time dependent stimulative effects on the proliferative activity of splenic lymphocytes and hepatocytes. GSP could promote the spleen lymphocytes and liver cells proliferation which cultured in vitro from Rat. So GSP had promoted effect to cellular antioxidant ability and immune function of the cells.