Abstract: The appropriate liquid fermentation medium were studied by measuring the mycelial biomass, the peloton density and the amount of germination vigor index and the single factor tests and orthogonal design method were employed to enhance the vitality of Pleurotus nebrodensis strains of liquid. The results showed that, the optimal suitable carbon source were corn flour, the optimum nitrogen source were soybean meal and peptone, the optimum medium were 3% of the corn flour, glucose1.5%, peptone 0.6%, soybean meal 2%.