Abstract: Researching the medium formula of Tremella cultivated by JUNCAO. Using test-tube method screened four kinds of JUNCAO and three auxiliary materials. The most suitable medium formula of Tremella cultivated by JUNCAO was: Neyraudia reynaudiana 70%, sorghum 5%, wheat bran 24%, lime 1%. Tremella mycelium grew fastest (1.067 cm/d). Fruit body budded earlier. Exhibition was superior to the contrast.