Abstract: The research aimed to analyze cold tolerance difference of Senecio cineraria and Heuchera micrantha, the scientific basis was provided for the cultivation and applies. Semi- lethal temperature was determined with the method of electric conductivity and Logistic the equation fitting S; the Pro was determined with the method of ninhydrin colorimety. The MDA was determined with the method of thiobarbituric acid colorimetric. The semi- lethal temperature of Senecio cineraria and Heuchera micrantha was - 17.38℃and -61.09℃. Under the different low-temperature treatment, the relative conductivity of Senecio cineraria was increased more zhan the relative conductivity of Heuchera micrantha. The contents of Pro in the leaves of Heuchera micrantha was lower than that of the control. The contents of MDA in the leaves of Senecio cineraria was much lower than the contents in the leaves of Heuchera micrantha under the different temperature treatment. The result with these two physiological indexes of the relative conductivity and the semi-lethal temperature consisted with the field observation. The cold resistance of Heuchera micrantha was more than the cold resistance of Senecio cineraria.