Abstract: In order to control the quality of apple polyphenol and identify the authenticity of apple juice products, the HPLC fingerprint of polyphenols in different sources of apple juice concentrate was established after optimizing the polyphenol analysis conditions and analyzing the type and content of polyphenols in apple juice concentrate from different sources. Using apple juice concentrate as a sample in this experiment, and under conditions of using Welchrom C18 column, at the detection wavelength of 280 nm, the column temperature 30℃, amount of sample 20μL, mobile phase of 0.3% three trifluoroacetic acid aqueous solution and methanol (flow 1 mL/min) and gradient elution, 12 common fingerprint peaks were determined. As well as the finger print similarity of polyphenol was above 0.900 and the content of polyphenol in 10 sources of apple juice concentrate was 5559 to 7971μg/mL. So the HPLC fingerprint of polyphenols for apple juice concentrate was initially established. This method can be used for the qualitative and quantitative analysis of apple polyphenols and will provide the theoretical basis for apple juice processing enterprises in their quality control of polyphenols.