Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2015, Vol. 31 ›› Issue (10): 142-147.doi: 10.11924/j.issn.1000-6850.casb15010139

Special Issue: 资源与环境 园艺 农业生态

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Analysis of Monosaccharide Compositions and Contents in Polysaccharide of Dendrobium officinale from Different Bionic Cultivation Modes

Xiao Qiang1, Yang Cong2, Zhang Zheng3, Zhou Dazhai1   

  1. (1Key Laboratory of Biological Resources Protection and Utilization of Hubei Province (Hubei Institutes for Nationalities), Enshi Hubei 445000;2School of Biological Science and Technology, Hubei University for Nationalities, Enshi Hubei 445000;3School of Forestry and Horticulture, Hubei University for Nationalities, Enshi Hubei 445000)
  • Received:2015-01-20 Revised:2015-03-18 Accepted:2015-02-12 Online:2015-05-05 Published:2015-05-05

Abstract: In order to make full use of the natural forest in southwest Hubei Province, the research to determine feasibility of bionic cultivated Dendrobium officinale under natural mixed forest was carried out. Five different planting patterns of bionic cultivation of Dendrobium officinale were studied. The content of polysaccharides and the monosaccharide composition of polysaccharides from Dendrobium officinale were analyzed by phenol-sulfuric acid method and pre-column derivatization HPLC. The results showed that the content of polysaccharides in the Dendrobium officinale ranged from 18% to 33%, of which the polysaccharides content of Dendrobium officinale cultured from Cunninghamia lanceolata was the highest. The polysaccharides of Dendrobium officinale were composed of mannose and glucose, of which the ratio of mannose and glucose ranged from 4.5 to 5.98, and all met the standard of Chinese Pharmacopoeia. However, the peak area ratio between mannose and glucose was significantly different. Faster polysaccharide accumulation capacity cultured from Cunninghamia lanceolata than other cultivation mode was found in the mixed forest of southwest Hubei Province in natural forest protection project. The study provided experimental evidence for the development of Dendrobium officinale utilizing natural mixed forest and understory rich moss resources in southwest Hubei Province.