Rooting Cultivation Medium for‘Gala’Apple Plantlet and Physiological Effect of SA on Transplanting

doi:10.11924/j.issn.1000-6850.casb15090140

Abstract

Abstract: Because rooting cultivation and transplanting of apple test- tube plantlets is difficult, and the survival rate is low, in this study,‘Gala’apple test-tube plantlets were taken as experiment materials, to investigate the effect of basic culture medium and three kinds of auxin and salicylic acid (SA), including indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and 1-naphthylacetic acid (NAA), on rooting and transplanting survival rate. The results showed that the basic medium, hormone type and concentration obviously influenced the rooting percentage of tube seedlings, 1/2MS was significantly better than the MS culture medium, IAA was superior to the other two kinds of auxins; exogenous SA could significantly increase the transplanting survival rate of tube seedlings and had the concentration effect. Appropriate‘Gala’rooting medium was 1/2MS IAA 0.2 mg/L, 0.8 mg/L salicylic acid treatment could significantly reduce the stomatal opening and significantly improve the survival rate of transplanting.

CLC Number:

S661

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