Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2016, Vol. 32 ›› Issue (36): 96-103.doi: 10.11924/j.issn.1000-6850.casb16020056

Special Issue: 生物技术 油料作物 园艺

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Cloning and Expression of BnSTO Encoding B-box-type Zinc-finger Proteins in Rapeseed

Hu Maolong, Long Weihua, Gao Jianqin, Zhang Jiefu, Chen Song, Pu Huiming   

  1. (Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Cotton and Rapeseed (Nanjing), Ministry of Agriculture/Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing 210014)
  • Received:2016-02-18 Revised:2016-12-11 Accepted:2016-04-22 Online:2016-12-26 Published:2016-12-26

Abstract: The B-box-type zinc finger proteins play crucial roles in regulating plant growth and development, and response to abiotic stresses in plants. In this paper, two genes BnSTO-1 and BnSTO-2, encoding B-box-type zinc finger proteins, were cloned from rapeseed (Brassica napus L.) by reverse transcription PCR (RT-PCR). The gene BnSTO-1 contained an open reading frame (ORF) of 723 bp, encoding a peptide of 240 amino acid residues. It was mapped on chromosome A8. The ORF of BnSTO-2 was 726 bp in length, encoding a peptide of 241 amino acid residues. It was mapped on chromosome C8. Sequence analysis of the genomic fragment demonstrated that the two BnSTOs genes consisted of three exons separated by two introns. BnSTO-1 and BnSTO-2 shared 96.68% amino acid sequence homology. Phylogenetic analysis revealed that deduced BnSTO-1 and BnSTO-2 proteins were close to AtSTO protein in Arabidopsis. Real-time quantitative PCR showed that BnSTO-1 and BnSTO-2 were both expressed in all tissues tested, with the highest expression in leaves and buds; their expressions were significantly increased at different time in treatments of salt, drought, and high temperature, suggesting BnSTO genes were putatively associated with the responses of abiotic stresses.

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