Abstract: By analyzing the content of inorganic elements in radix pseudostellariae from Guizhou, we aim to assess the present quality of radix pseudostellariae, set a limited standard of heavy metals, establish an element finger-print, and find out the characteristic elements. The content of inorganic elements was measured and an element finger-print was drawn by the ICP-MS. Additionally, WM/T2-2004 was applied to evaluating the quality of heavy metal elements, and the characteristic elements were determined by principal component analysis. The results showed that the content of inorganic elements in radix pseudostellariae was between 0.057 and 959 mg/kg, the coefficient of variation was 0.134 -1.478, and the content of Cd, As, Pb, and Hg was below the Standard of WM/T2-2004 in 90% radix pseudostellariae. The limited standard of heavy metals in radix pseudostellariae was Cr≤6.5 mg/kg, Cu≤10 mg/kg, As≤2.0 mg /kg, Cd≤0.3 mg/kg, Hg≤0.15 mg/kg, Pb≤4.0 mg/kg. The features of the inorganic elements finger- print could provide theoretical basis in identifying the quality of radix pseudostellariae and distinguish the radix pseudostellariae from other Chinese herbal medicines. The characteristic inorganic elements of radix pseudostellariae were found to be Cd, Cu, Co, Zn, Fe, Ca, Mg, and Al. Radix pseudostellariae from Guizhou contained abundant inorganic elements, the content of heavy metals was below the evaluation criterion, the limited standard of heavy metals was proposed, the element finger-print was drawn, and the characteristic elements were determined eventually.