Abstract: Seven SSR primers were used to identify 7 rice and maize distant hybrid posterity indica rice PTGMS lines. In comparison with female parents, 18 polymorphic bands are specific bands,namely SSR polymorphism.In the specific bands, 13 bands were identical with the male parent,s, and the other 5 bands were variant from the parent’s are variant bands. All of the posterity indica rice PTGMS lines were detected of SSR polymorphism, but the quantity of sites being detected is variant between the lines. The most one is ys-2, in which three SSR polymorphic sites were got. It was preliminarily proved that some DNA segments of maize might have been transferred into rice PTGMS lines by rice and maize distant hybrid, and the improvement of indica rice PTGMS lines had been succeeded preliminarily.