RES Addition in IVM Medium Affects Development and Anti-freezing Ability of Porcine MII Stage Oocytes in Vitro

doi:10.11924/j.issn.1000-6850.casb17070057

Abstract

Abstract: The purpose of this study was to clarify the effect of RES addition in IVM medium on in vitro development and anti-freezing ability of porcine oocytes. Different concentrations of RES were added in IVM media, the in vitro developmental ability and the expression levels of related genes were analyzed. After open pulled straw (OPS) vitrification and thawing, the mitochondrial membrane potential (ΔΨm), early stage apoptotic level and parthenogenetic developmental ability of porcine oocytes were analyzed. The results were as follows. (1) 2 μmol/L RES addition could improve the IVM rate and parthenogenetic cleavage rate, a high concentration of RES addition (10 μmol/L) showed a cytotoxic effect on the developmental ability; (2) 2 μmol/L RES addition in IVM medium significantly improved the expression levels of Sirt1, MATER, SOD1 and Bcl2, and decreased the expression levels of c-mos, MAPK and Mfn2; when the RES was 10 μmol/L, the expression of apoptotic related genes presented proapoptotic status; (3) ROS levels in fresh or vitrified oocytes of RES addition groups were lower than that of control group, ATP contents and ΔΨm values were higher than those of control group without RES addition; (4) RES addition in IVM medium increased the FDA survival and parthenogenetic cleavage rates, and decreased the early stage apoptotic rate than those of control group without RES addition. In conclusion, 2 μmol/L RES addition in porcine IVM medium can lead to better gene expression levels, increase mitochondrial function, then improve the development and anti-freezing ability of porcine oocytes

References

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