Abstract: Abstract: In order to improve the system for embryo culture in vitro. In vitro fertilization embryos were used as research models, screening and optimizing the various factors that affect IVF embryo development in vitro, such as sperm capacitation medium, embryo culture medium, the concentration of serum and BSA and cumulus cells and embryo co-culture. Our results showed that the the IVF embryos cultured in BO capacitation medium obtain higher cleavage rate than that cultured in normal capacitation medium. Added glucose and, BSA and FBS to embryo culture medium obtained better embryo development effect, the cleavage rate and blastocyst rate reached (79.85±0.74)% and (28.69±1.45)% respectively. On this basis, the blastocyst rate increased to (32.70±1.73)% in bovine cumulus cells and embryos co-culture，The results of immunofluorescence staining showed that the bovine cumulus cells was not apoptotic during co-culture. This study further optimized the bovine embryo development system in vitro and improved the rate of blastocyst in vitro culture.