Abstract: The paper aims to clarify the temporal dynamics and structure of insect community in the rotation field of flue-cured tobacco and oilseed rape, and provide a reference for pest integrated management of fluecured tobacco. In 2016, the insect species and numbers were investigated by Malaise trap in the rotation field of flue-cured tobacco and oilseed rape in Yuxi City. The results showed that a total of 5089 insect samples were collected and the insects belonged to 9 orders, 26 taxa. Leafhopper and phalaenae were the dominant species of phytophagous insects. Ichneumon and braconidae were the dominant species of parasitic insects. Predaceous syrphidae was the dominant species of predacious insects. Population peak of phalaenae was from June to July, population peak of leafhopper was September. Population peak of ichneumon was September. Population peak of braconidae was June. Population peak of predaceous syrphidae was from April to May. The diversity index, richness index and evenness index reached peak in June in the rotation field, the maximum was 1.9850, 2.7620 and 1.0000, respectively. The dominance index reached peak in March, the maximum was 0.8840. Ss/Si (the number of species divided by the number of individuals) was between 0.0088 and 0.1385, Sn/Sp (the number of natural enemy species divided by the number of phytophagous species) was between 0.7142 and 0.2000. Ss/Si index and Sn/Sp index reached peak in August. The structure of insect community was the most stable from June to August.