Abstract: Hormone is a critical factor that affects the multiplication and vitrification of carnation shoots.In our expriment ,orthogonal test L9(34) was applied to study the effect of hormones BA、NAA、KT conbination on the multiplication ,and carnation shoots were used as explants. The results showed that BA was the determinate factor for multiplication and vitrification of carnation shoots, and NAA was followed, KT had no positive effect on multiplication of carnation shoots, but the least effect on vitrification. The multiplication of carnation shoots was not only related to the concentration of BA, but to the ration of BA and NAA.The medium for the shoot differentiation and subculture were optimized.