Mannanase Production by Lactobacillus casei HDS-01: Condition Study

doi:10.11924/j.issn.1000-6850.casb20190900618

Abstract

Abstract:

The purpose of this research is to increase the mannanase production of Lactobacillus casei HDS-01 isolated from the fermentation liquid of pickled Chinese cabbage and lay a foundation for the separation, purification and industrial production of mannanase. The single factor experiment was carried out by setting different medium components and fermentation conditions based on MRS. Mannanase activity and L.casei HDS-01 growth were measured by DNS method and flat colony counting method, respectively. The results showed that the optimum condition for mannanase production by L. casei HDS-01 is: 0.8% pectin as optimum carbon source, 0.2% yeast extract and ammonium nitrate as optimum nitrogen source, 0.2% ammonium citrate, 0.1% dipotassium hydrogenphosphate and 0.9% sodium acetate as inorganic salts; 100 mL /250 mL liquid loading, 7% inoculation, 37℃ culture temperature and initial pH value of 5.5. Under the optimal condition, after 36 h of shaking at a speed of 140 r/min, the enzyme activity could reach 72.7±0.30 U/mL, which is 6.51 times higher than that before optimization.

Key words: Lactobacillus casei, lactic acid bacteria, mannanase, medium component, fermentation condition

CLC Number:

Q935

Zhang Xin, Wang Yao, Jiang Jing, Zhao Dan. Mannanase Production by Lactobacillus casei HDS-01: Condition Study[J]. Chinese Agricultural Science Bulletin, 2020, 36(29): 78-86.

Figures/Tables 9

References 26

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因素种类及水平	酶活力/(U/mL)	优化倍数	多重比较结果
碳氮比：4:1	72.7±0.30	6.51	A
0.2%硝酸铵	67.2±0.12	5.92	B
7%接种量	65.7±0.05	5.77	C
1.0%葡萄糖	64.4±0.39	5.63	C
培养基初始pH 5.5	50.6±0.58	4.21	C
摇床转速：140 r/min	44.2±0.40	3.55	DE
培养温度：37℃	42.4±0.64	3.37	E
装液量：100 mL/250 mL	40.9±0.22	3.22	EF
0.9%乙酸钠	38.7±0.88	2.98	F
0.1%K₂HPO₄	34.8±0.23	2.58	F
0.2%柠檬酸铵	33.8±0.71	2.48	F
0.2%酵母提取物	32.2±0.30	2.31	F
0.8%果胶	28.1±0.39	1.89	G
对照(MRS)	9.7±0.14	0	G

因素种类及水平	酶活力/(U/mL)	优化倍数	多重比较结果
碳氮比：4:1	72.7±0.30	6.51	A
0.2%硝酸铵	67.2±0.12	5.92	B
7%接种量	65.7±0.05	5.77	C
1.0%葡萄糖	64.4±0.39	5.63	C
培养基初始pH 5.5	50.6±0.58	4.21	C
摇床转速：140 r/min	44.2±0.40	3.55	DE
培养温度：37℃	42.4±0.64	3.37	E
装液量：100 mL/250 mL	40.9±0.22	3.22	EF
0.9%乙酸钠	38.7±0.88	2.98	F
0.1%K₂HPO₄	34.8±0.23	2.58	F
0.2%柠檬酸铵	33.8±0.71	2.48	F
0.2%酵母提取物	32.2±0.30	2.31	F
0.8%果胶	28.1±0.39	1.89	G
对照(MRS)	9.7±0.14	0	G