The Insolationan and Screening of A High Lactate-producing Lactic Acid Bacterium

doi:10.11924/j.issn.1000-6850.casb16040180

Abstract

Abstract: Lactate is widely used in food, textile, chemical and pharmaceutical industries as a natural organic acid. A high lactate-producing bacterium, strain 11MZ-5-1 was isolated from the liquid sample of pickle Chinese cabbage. The concentration of lactate produced by strain 11MZ-5-1 was 24.88±0.21 g/L at 72 h. The strain was identified according to morphological, physiological and biochemical methods. Meanwhile, the phylogenetic tree was conducted based on its partial 16S rDNA sequence. The results showed that strain 11MZ-5-1 could grow at 20-45℃, with the optimum temperature range 30-37℃. The salt-tolerance range was 0-12%. The tolerance of pH was 3.5-7.5 with the optimum pH was 5.5 to 6.5. Its partial sequence of the 16S rDNA was 1438 bp which was 99% similar with Lactobacillus. casei str. Zhang. Strain 11MZ-5-1 was identified as L. casei. The results suggested its great application potential in industrial production due to the short fermentation period and high lactate production.

CLC Number:

平文祥,,,, and . The Insolationan and Screening of A High Lactate-producing Lactic Acid Bacterium[J]. Chinese Agricultural Science Bulletin, 2017, 33(7): 41-47.

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