Screening, Identification and Characteristic Analysis of Cellulase-Producing Bacteria

doi:10.11924/j.issn.1000-6850.casb2020-0855

Abstract

Abstract:

To screen cellulase-producing bacteria and provide a material basis for the development of new feed additives, in this study, soil samples from corn fields were used to screen cellulase-producing bacteria by congo red plate method, and the characteristics of cellulase produced by it were analyzed. Through the test, the cellulase-producing strain was screened and identified as Bacillus megaterium by morphological and molecular biological methods. The enzymatic characteristics of cellulase produced by Bacillus megaterium XT2 was analyzed and the optimal reaction condition was found to be 50℃ and pH 6.0, and the cellulase showed certain thermal stability, K⁺ could activate the cellulase activity, and Mg²⁺, Ca²⁺ and Mn²⁺ had an inhibitory effect on enzyme activity. When the strain grew to 20 h, the cellulase activity reached the highest (0.774 U/mL). The successful isolation of cellulase-producing Bacillus could provide a strain material for the development of new feed resources and new feed additives.

Key words: cellulase, Bacillus, screening, identification, enzymatic characteristics

CLC Number:

Zhao Longmei, Chen Lin, Du Dongxiao, Dong Huixin, Li Wang, Li Yuanxiao, He Wanling, Cao Pinghua. Screening, Identification and Characteristic Analysis of Cellulase-Producing Bacteria[J]. Chinese Agricultural Science Bulletin, 2021, 37(30): 83-88.

Figures/Tables 9

References 26

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