Effects of Different Hosts on Propagation of Arbuscular Mycorrhizal Fungi

doi:10.11924/j.issn.1000-6850.casb2021-0050

Abstract

Abstract:

Arbuscular mycorrhizal fungi (AMF) play an important role in soil ecosystem, but currently AMF cannot be purely cultured, and can only be propagated by symbiosis with host plants. In order to research the effect of different host plants on the propagation of AMF, maize (Zea mays L.), sorghum (Sorghum bicolor (L.) Moench), ryegrass (Lolium perenne L.) and clover (Trifolium pratense L.) were selected as hosts to propagate Rhizophagus aggregatus and Claroideoglomus etunicatum, respectively. The colonization rate, spore density, and inoculum potential of AMF were measured on the 30^th, 60^th, 90^th and 120^th day after AMF inoculation. The results showed that the effects of different hosts on the propagation of AMF were different. The colonization rates of R. aggregatus and C. etunicatum from high to low were maize > sorghum > clover > ryegrass, and the order of spore density of R. aggregatus was maize> sorghum > ryegrass > clover, the order of spore density of C. etunicatum was sorghum > maize> clover > ryegrass. The propagation effect of the same host on different AMF was also different. The effect of maize and ryegrass on R. aggregatus was higher than that of C. etunicatum, but the effect of clover and sorghum on the propagation of C. etunicatum was higher than that of R. aggregatus. It was also found that the spore density of AMF was related to the colonization rate of AMF, and there was a significantly positive correlation. The results show that maize is the best host plant for R. aggregatus propagation and sorghum is the best host plant for C. etunicatum propagation.

Key words: arbuscular mycorrhizal fungi, propagation, colonization rate, spore density, host plants, inoculum potential

CLC Number:

Q939.96

MA Junqing, HOU Ning, SUN Chenyu, YANG Yisen, QIN Shengfeng, WANG Yong, LIU Lu, LIAO Honglin, HUANG Jinghua. Effects of Different Hosts on Propagation of Arbuscular Mycorrhizal Fungi[J]. Chinese Agricultural Science Bulletin, 2022, 38(1): 7-14.

Figures/Tables 7

References 28

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宿主名称	宿主基本情况	品种	来源
玉米	一年生禾本科草本植物,单子叶C4植物,须根系	正大619	四川正大种业提供
三叶草	多年生豆科牧草,双子叶C3植物,直根系	未知	甘肃农业大学草业学院提供
黑麦草	多年生禾本科牧草,单子叶C3植物,须根系	维多利亚	河南世纪天缘生态科技有限公司提供
高粱	一年生禾本科草本植物,单子叶C4植物,须根系	未知	自山东高密农民手中购得

宿主名称	宿主基本情况	品种	来源
玉米	一年生禾本科草本植物,单子叶C4植物,须根系	正大619	四川正大种业提供
三叶草	多年生豆科牧草,双子叶C3植物,直根系	未知	甘肃农业大学草业学院提供
黑麦草	多年生禾本科牧草,单子叶C3植物,须根系	维多利亚	河南世纪天缘生态科技有限公司提供
高粱	一年生禾本科草本植物,单子叶C4植物,须根系	未知	自山东高密农民手中购得

AMF	宿主植物	初始孢子密度/(个/g)	终孢子密度/(个/g)	扩繁效果/%
RA	玉米	24	78.13±3.50Aa	325.56±14.57Aa
	三叶草		54.67±5.51Bb	227.78±22.96Bb
	黑麦草		71.07±2.22ABa	296.11±9.285ABa
	高粱		72.27±2.63Aa	301.11±10.94Aa
	差异显著性		*
	F值		7.444
	P值		0.0106
CE	玉米	26	71.87±2.83ABab	276.41±10.85ABab
	三叶草		67.60±1.60Bbc	260.00±6.15Bbc
	黑麦草		63.33±3.14Bc	243.59±12.09Bc
	高粱		79.87±1.96Aa	307.18±7.55Aa
	差异显著性		**
	F值		8.201
	P值		0.008

AMF	宿主植物	初始孢子密度/(个/g)	终孢子密度/(个/g)	扩繁效果/%
RA	玉米	24	78.13±3.50Aa	325.56±14.57Aa
	三叶草		54.67±5.51Bb	227.78±22.96Bb
	黑麦草		71.07±2.22ABa	296.11±9.285ABa
	高粱		72.27±2.63Aa	301.11±10.94Aa
	差异显著性		*
	F值		7.444
	P值		0.0106
CE	玉米	26	71.87±2.83ABab	276.41±10.85ABab
	三叶草		67.60±1.60Bbc	260.00±6.15Bbc
	黑麦草		63.33±3.14Bc	243.59±12.09Bc
	高粱		79.87±1.96Aa	307.18±7.55Aa
	差异显著性		**
	F值		8.201
	P值		0.008

宿主植物	AMF	扩繁效果/%	宿主植物	AMF	扩繁效果/%
玉米	RA	325.56±14.57	黑麦草	RA	296.11±9.25
	CE	276.41±10.85		CE	243.59±12.09
	差异显著性	ns		差异显著性	*
	F值	1.8023		F值	1.7103
	P值	0.0538		P值	0.026
	t值	2.7047		t值	3.4503
三叶草	RA	227.78±22.96	高粱	RA	301.11±10.94
	CE	260.00±6.15		CE	307.18±7.55
	差异显著性	ns		差异显著性	ns
	F值	13.9204		F值	2.0984
	P值	0.2467		P值	0.6718
	t值	1.3556		t值	1.4564