Effect of FSH on the Expression of NGF and Its Receptor TrkA in the Testicles of Males After Sexual Maturity

doi:10.11924/j.issn.1000-6850.casb2020-0258

Abstract

Abstract:

In this experiment, we studied the expression of nerve growth factor (NGF) and its receptor tyrosine kinase (TrkA) in the testis of sexually mature roosters, and the influence of FSH in the testis on TrkA mRNA expression. Twenty-eight Zhuanghe Big Bone Chicken roosters aged twenty weeks were selected and randomly divided into 4 groups, namely the control group and three FSH treatment groups. In the treatment group, FSH was intravenously injected for five days, and testicular tissue was collected on the sixth day. The results showed that there were NGF and TrkA mRNA and protein in rooster testis tissue, and both of them were mainly expressed in spermatogonia, spermatocytes and sperm cells; TrkA mRNA levels in the FSH treatment group were significantly higher than those in the control group (P<0.05), and the expression level of TrkA mRNA increased with the increase of FSH concentration, but there was no significant difference in the expression level of NGF mRNA (P>0.05). In summary, the above results indicate that there are NGF and TrkA in rooster testis tissue, and FSH may affect the role of NGF in sperm production by regulating the expression of TrkA.

Key words: follicle stimulating hormone, cock testicle, nerve growth factor, tyrosine kinase receptor A

CLC Number:

S831.2

Wang Jinfeng, Lu Yi, Dai Tianshu, Zhang Jiehui, Wang Shuo, Zou Shuang, Ma Wei, Wang Chunqiang. Effect of FSH on the Expression of NGF and Its Receptor TrkA in the Testicles of Males After Sexual Maturity[J]. Chinese Agricultural Science Bulletin, 2020, 36(36): 118-124.

Figures/Tables 5

References 35

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基因	引物	引物序列(5＇-3＇)	扩增长度/bp	参考
NGF	Forward	AGAGTAACGGACAGCACATT	146	NM_001293108
NGF	Reverse	CAGGACCCGAGAAGACC	146	NM_001293108
TrkA	Forward	CGTGTCCCTCCGAGGTGTATG	226	NM_205378
TrkA	Reverse	AGCGTCAGGGCATCAGTCC	226	NM_205378
Beta-actin	Forward	CTGTGCCCATCTATGAAGGCTA	139	NM_205518
Beta-actin	Reverse	ATTTCTCTCTCGGCTGTGGTG	139	NM_205518

基因	引物	引物序列(5＇-3＇)	扩增长度/bp	参考
NGF	Forward	AGAGTAACGGACAGCACATT	146	NM_001293108
NGF	Reverse	CAGGACCCGAGAAGACC	146	NM_001293108
TrkA	Forward	CGTGTCCCTCCGAGGTGTATG	226	NM_205378
TrkA	Reverse	AGCGTCAGGGCATCAGTCC	226	NM_205378
Beta-actin	Forward	CTGTGCCCATCTATGAAGGCTA	139	NM_205518
Beta-actin	Reverse	ATTTCTCTCTCGGCTGTGGTG	139	NM_205518