Abstract: The experiments about tissue culture of Garlic shoot-tip were made in MS mediums supplemented with different phytohormones. And then the best medium for rapid propagation of Garlic was obtained. The results were as follows: the optimized medium for inducing callus was MS+BA 0.2mg/L+NAA0.5mg/L, and the growth rate of callus was 60%. The suitable medium for inducing adventitious buds was MS +BA1.0 mg/L+NAA 0.1mg/L, and the highest propagatiion rate reached 80%. At the same time, the effects of different phytohormones, pH and glutamate on the propagation coefficient in Garlic have been researched. Then the results indicated: Propagation coefficient is raised to 13.8 in the medium supplemented with 0.5mg/L GA3. And it also can be raised in pH5.8. Propagation coefficient in subculture is remained at high level in 10mg/L glutamate.