Effect of Chlorella on the Quality and Bacterial Community Structure of GIFT Tilapia Culture Water

doi:10.11924/j.issn.1000-6850.casb2020-0287

Abstract

Abstract:

By adding Chlorella to tilapia aquaculture water, this study aims to explore the optimal concentration of Chlorella for the degradation of N and P and the regulation of microbial community in aquaculture water. Control group (NC), low concentration group (LC), middle concentration group (MC) and high concentration group (HC) were set to explore the effects of different concentrations of Chlorella on reducing the concentration of N and P in tilapia aquaculture water. The 16S rRNA high-throughput sequencing technology was used to analyze bacteria community structure changes of aquaculture water body. Compared with other treatment groups, N and P nutrition of water body in HC group decreased, and the degradation rate of nitrogen in three forms was NH₄ ⁺-N>NO₃^--N>NO₂^--N. Compared with NC group, the microbial diversity of MC and HC group decreased. The relative abundance of Proteobacteria in each treatment group increased, while that of Bacteroidetes decreased, and the relative abundance of Bacteroidetes in HC group was the highest, the community richness was significantly correlated with the content of NH₄⁺-N. In summary, Chlorella can absorb N and P nutrition from aquaculture water, regulate aquaculture water microbial community structure, and improve aquaculture water quality. The study could provide a theoretical basis for aquaculture production practice.

Key words: Chlorella, tilapia, aquaculture water, water quality, the degradation of N and P, high-throughput sequencing, bacterial community structure

CLC Number:

Qin Lu, Chen Xi, Qiu Liping, Fan Limin, Song Chao, Zheng Yao, Meng Shunlong, Chen Jiazhang. Effect of Chlorella on the Quality and Bacterial Community Structure of GIFT Tilapia Culture Water[J]. Chinese Agricultural Science Bulletin, 2021, 37(12): 112-120.

Figures/Tables 7

References 35

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处理组	Sobs指数	Shannon指数	Simpson指数	Ace指数	Chao指数
NC1	303±36.37^b	3.35±0.04^b	0.07±0^a	504.71±122.56^b	435.2±68.31^b
LC1	343.67±20.74^b	3.39±0.03^b	0.08±0.01^a	646.2±112.33^ab	525.92±60.67^b
MC1	531.33±51.54^a	3.87±0.17^a	0.05±0.01^a	757.05±115.8^a	719.21±69.41^a
HC1	466.33±32.13^a	3.65±0.16^a	0.08±0.03^a	782.39±60.4^a	680.92±41.08^a
NC2	430.33±35.13^a	3.61±0.52^a	0.08±0.05^a	581.29±35.06^a	547.28±25.5^a
LC2	456.67±22.3^a	3.73±0.18a	0.08±0.02^a	639.14±121.65^a	637.88±99.54^a
MC2	450±17.69^a	3.73±0.2^a	0.06±0.02^a	675.97±69.92^a	629.97±23.33^a
HC2	452±11.53^a	3.64±0.24^a	0.08±0.04^a	704.15±80.72^a	645.06±51.33^a
NC3	499±50.69^a	3.77±0.52^a	0.07±0.06^a	689.81±123.04^a	672.78±79.1^a
LC3	484.33±21.94^a	4.02±0.26^a	0.05±0.02^a	637.07±9.29^a	651.47±5^a
MC3	475±5.29^a	3.8±0.08^a	0.07±0.01^a	646.9±52.8^a	625.38±14.62^a
HC3	495.33±100.57^a	3.63±1.08^a	0.14±0.19^a	643.83±132.16^a	654.59±135.61^a
NC4	459.67±21.13^a	3.74±0.15^a	0.08±0.01^a	593.19±16.82^a	597.37±32.2^a
LC4	441.33±27.75^a	3.85±0.16^a	0.05±0.01^a	567.98±37.69^a	577.98±31.37^ab
MC4	420.67±65.01^a	3.94±0.4^a	0.05±0.03^a	536.46±60.73^a	529.67±50.66^b
HC4	461.67±9.71^a	3.89±0.17^a	0.06±0.01^a	586.96±13.68^a	591.98±20.77^a

处理组	Sobs指数	Shannon指数	Simpson指数	Ace指数	Chao指数
NC1	303±36.37^b	3.35±0.04^b	0.07±0^a	504.71±122.56^b	435.2±68.31^b
LC1	343.67±20.74^b	3.39±0.03^b	0.08±0.01^a	646.2±112.33^ab	525.92±60.67^b
MC1	531.33±51.54^a	3.87±0.17^a	0.05±0.01^a	757.05±115.8^a	719.21±69.41^a
HC1	466.33±32.13^a	3.65±0.16^a	0.08±0.03^a	782.39±60.4^a	680.92±41.08^a
NC2	430.33±35.13^a	3.61±0.52^a	0.08±0.05^a	581.29±35.06^a	547.28±25.5^a
LC2	456.67±22.3^a	3.73±0.18a	0.08±0.02^a	639.14±121.65^a	637.88±99.54^a
MC2	450±17.69^a	3.73±0.2^a	0.06±0.02^a	675.97±69.92^a	629.97±23.33^a
HC2	452±11.53^a	3.64±0.24^a	0.08±0.04^a	704.15±80.72^a	645.06±51.33^a
NC3	499±50.69^a	3.77±0.52^a	0.07±0.06^a	689.81±123.04^a	672.78±79.1^a
LC3	484.33±21.94^a	4.02±0.26^a	0.05±0.02^a	637.07±9.29^a	651.47±5^a
MC3	475±5.29^a	3.8±0.08^a	0.07±0.01^a	646.9±52.8^a	625.38±14.62^a
HC3	495.33±100.57^a	3.63±1.08^a	0.14±0.19^a	643.83±132.16^a	654.59±135.61^a
NC4	459.67±21.13^a	3.74±0.15^a	0.08±0.01^a	593.19±16.82^a	597.37±32.2^a
LC4	441.33±27.75^a	3.85±0.16^a	0.05±0.01^a	567.98±37.69^a	577.98±31.37^ab
MC4	420.67±65.01^a	3.94±0.4^a	0.05±0.03^a	536.46±60.73^a	529.67±50.66^b
HC4	461.67±9.71^a	3.89±0.17^a	0.06±0.01^a	586.96±13.68^a	591.98±20.77^a