Virulence Factors of Pathogenic Fusarium oxysporum: Research Progress

doi:10.11924/j.issn.1000-6850.casb2020-0389

Abstract

Abstract:

Plant wilt disease triggered by pathogenic Fusarium oxysporum infecting plant is a devastating soil borne fungal disease that is widespread in the world, and the fungi are currently ranked as the fifth most phytopathogenic fungi in the world. Because the pathogen is transmitted through the soil, its pathogenic mechanism has not been fully defined, and a full understanding of the pathogenic mechanism of F. oxysporum is a prerequisite for combating this disease. To better explore and implement the effective control strategies and thus limit host plant infection, this review concludes the pathogenesis of F. oxysporum, summarizes the contribution of cell wall degrading enzymes, toxins, signaling pathways (MAPK pathways) in virulence factors to the pathogenic process, and analyzes the regulatory mechanisms of virulence genes and transcription factors during infection. It is hoped to provide targets and rationale for controlling wilt diseases caused by F. oxysporum.

Key words: Fusarium oxysporum, plant wilt, soil borne fungal disease, phytopathogenic fungi, cell wall degrading enzyme, toxin, virulence gene, transcription factor

CLC Number:

S432.1

Li Ying, Du Chunmei. Virulence Factors of Pathogenic Fusarium oxysporum: Research Progress[J]. Chinese Agricultural Science Bulletin, 2021, 37(12): 92-97.

Figures/Tables 1

References 50

[1]	肖荣凤, 刘波, 朱育菁, 等. 非致病性尖孢镰刀菌FJAT-9290的定殖特性及对番茄枯萎病的防治效果[J]. 植物保护学报, 2015,42(02):169-175.
[2]	Dean R, Kan J A, Pretorius Z A, et al. The top 10 fungal pathogens in molecular plant pathology[J]. Molecular Plant Pathology, 2012,13(7):414-430. doi: 10.1111/j.1364-3703.2011.00783.x URL
[3]	Zhang Y, Li J M. Fungal phylogenetics and phylogenomics volume 100 deciphering pathogenicity of Fusarium oxysporum from a phylogenomics perspective[J]. Advances in Genetics, 2017,9(19):179-209.
[4]	Kanani P, Shukla Y M. Genetic variability: physiological characteristics, pathogenicity and molecular diversity of Fusarium oxysporum f.sp. cumini infecting Cumin cyminum L. in India[J]. Scientific Reports, 2020,33(2):265-276.
[5]	Swarupa V, Ravishankar K V, Rekha A. Plant defense response against Fusarium oxysporum and strategies to develop tolerant genotypes in banana[J]. Planta, 2014,239(4):735-751. doi: 10.1007/s00425-013-2024-8 pmid: 24420701
[6]	Sharma M, Sengupta A, Ghosh R, et al. Genome wide transcriptome profiling of Fusarium oxysporum f.sp. ciceris conidial germination reveals new insights into infection-related genes[J]. Scientific Reports, 2016,6(37353).
[7]	Li C Q, Shao J F, Wang Y J, et al. Analysis of banana transcriptome and global gene expression profiles in banana roots in response to infection by race 1 and tropical race 4 of Fusarium oxysporum f.sp.cubense[J]. Bmc Genomics, 2013,14(1):851. doi: 10.1186/1471-2164-14-851 URL
[8]	Caffall K H, Mohnen D. The structure, function and biosynjournal of plant cell wall pectic polysaccharides[J]. Carbohydrate Research, 2009,344(14):1879-1900. doi: 10.1016/j.carres.2009.05.021 pmid: 19616198
[9]	Glass N L, Schmoll M, Cate J H D, et al. Plant cell wall deconstruction by Ascomycete fungi[J]. Annual Review of Microbiology, 2013,67(1):477-498. doi: 10.1146/annurev-micro-092611-150044 URL
[10]	Pedro L, Carmen R R, Concepcion H. Role of the phosphatase Ptc1 in stress responses mediated by CWI and HOG pathways in Fusarium oxysporum[J]. Fungal Genetics and Biology, 2018,118:10-20. doi: 10.1016/j.fgb.2018.05.004 URL
[11]	Jones T M, Anderson A J, Albersheim P. Host-pathogen interactions IV studies on the polysaccharide-degrading enzymes secreted by Fusarium oxysporum f.sp. lycopersici[J]. physiological plant pathology, 1972,2(2):153-166. doi: 10.1016/0048-4059(72)90023-9 URL
[12]	Ruiz G B, Pietro A D, Roncero M I G. Combined action of the major secreted exo and endo polygalacturonases is required for full virulence of Fusarium oxysporum[J]. Molecular Plant Pathology, 2016,17(3):53-339.
[13]	Medina M A R, Sanchez K L M. GTPase Rho1 regulates the expression of xyl3 and laccase genes in Fusarium oxysporum[J]. Biotechnology Letters, 2015,37(3):679-683. doi: 10.1007/s10529-014-1709-9 URL
[14]	艾聪聪, 惠金聚, 王桂清, 等. 尖孢镰刀菌细胞壁降解酶活性研究[C]. 中国植物病理学会, 2017: 85-89.
[15]	Dor E, Evidente A, Amalfitano C, et al. The influence of growth conditions on biomass, toxins and pathogenicity of Fusarium oxysporum f.sp.orthoceras, a potential agent for broomrape biocontrol[J]. Weed Research, 2007,47(4):345-352. doi: 10.1111/wre.2007.47.issue-4 URL
[16]	Nirmaladevi D, Venkataramana M, Srivastava R K, et al. Molecular phylogeny, pathogenicity and toxigenicity of Fusarium oxysporum f.sp.lycopersici[J]. Entific Reports, 2016,6:21367.
[17]	Bouizgarne B, Bouteau H E M, Frankart C, et al. Early physiological responses of Arabidopsis thaliana cells to fusaric acid:toxic and signalling effects[J]. New Phytologist, 2006,169(1):209-218. pmid: 16390432
[18]	Wu Z J, Liu Y, Wang R Y, et al. In vitro study of the growth, development and pathogenicity responses of Fusarium oxysporum to phthalic acid, an autotoxin from Lanzhou lily[J]. World Journal of Microbiology & Biotechnology, 2015,35:123-145. doi: 10.1007/s11274-019-2699-5 URL
[19]	Wu Z J, Xie Z K, Yang L, et al. Identification of autotoxins from root exudates of Lanzhou lily (Lilium davidii var. unicolor)[J]. Allelopathy Journal, 2015,35(1):35-48.
[20]	Irzykowska L, Bocianowski J, Agnieszka W, et al. Genetic variation of Fusarium oxysporum isolates forming fumonisin B1 and moniliformin[J]. Journal of Applied Genetics, 2012,53(4):237-247. doi: 10.1007/s13353-012-0087-z URL
[21]	Jiang X F, Qiao F, Long Y L, et al. MicroRNA-like RNAs in plant pathogenic fungus Fusarium oxysporum f.sp.niveum are involved in toxin gene expression fine tuning[J]. Biotech, 2017,7(5):354.
[22]	Covarelli L, Beccari G, Prod A, et al. Biosynjournal of beauvericin and enniatins in vitro by wheat Fusarium species and natural grain contamination in an area of central Italy[J]. Food Microbiology, 2015,46(6):618-626. doi: 10.1016/j.fm.2014.09.009 URL
[23]	Moretti A, Belisario A, Tafuri A, et al. Production of beauvericin by different races of Fusarium oxysporum F.sp.melonis, The Fusarium Wilt Agent of Muskmelon[J]. European Journal of Plant Pathology, 2002,3(7):354.
[24]	Husaini A M, Sakina A, Cambay S R. Host-pathogen interaction in Fusarium oxysporum infections: where do we stand[J]. Molecular Plant Microbe Interactions Mpmi, 2018,31(9):889-898. doi: 10.1094/MPMI-12-17-0302-CR URL
[25]	Luque D S, Pietro A D, Nadales E P, et al. Three Fusarium oxysporum mitogen-activated protein kinases (MAPKs) have distinct and complementary roles in stress adaptation and cross-kingdom pathogenicity[J]. Molecular Plant Pathology, 2017,18(7):345-456.
[26]	Nadales E P, Di Pietro A D. The transmembrane protein Sho1 cooperates with the mucin Msb2 to regulate invasive growth and plant infection in Fusarium oxysporum[J]. Molecular Plant Pathology, 2015,16(6):593-603. doi: 10.1111/mpp.2015.16.issue-6 URL
[27]	Martínez R A L, Roncero M I G, Marine M, et al. Rho1 has distinct functions in morphogenesis cell wall biosynjournal and virulence of Fusarium oxysporum[J]. Cellular Microbiology, 2008,10(6):1339-1351. doi: 10.1111/j.1462-5822.2008.01130.x URL
[28]	Jane S, Akiyama K, Takata R, et al. Signaling via the G protein α subunit FGA2 is necessary for pathogenesis in Fusarium oxysporum[J]. FEMS Microbiology Letters, 2017,243(1):72-165.
[29]	Pietro A D, Garcia M F I, Meglecz E, et al. A MAP kinase of the vascular wilt fungus Fusarium oxysporum is essential for root penetration and pathogenesis[J]. Molecular Microbiology, 2010,39(5):1140-1152. doi: 10.1111/j.1365-2958.2001.02307.x URL
[30]	Rojas C M, Hera A C. The F-box protein Fbp1 functions in the invasive growth and cell wall integrity mitogen-activated protein kinase (MAPK) pathways in Fusarium oxysporum[J]. Molecular Plant Pathology, 2016,17(1):55-64. doi: 10.1111/mpp.12259 URL
[31]	Rispail N, Pietro A D. The two-component histidine kinase Fhk1 controls stress adaptation and virulence of Fusarium oxysporum[J]. Molecular Plant Pathology, 2010,11(3):395-407. doi: 10.1111/mpp.2010.11.issue-3 URL
[32]	Pareek M, Rajam M V. RNAi-mediated silencing of MAP kinase signalling genes (Fmk1,Hog1, and Pbs2) in Fusarium oxysporum reduces pathogenesis on tomato plants.[J]. Fungal Biology, 2017,121(9):775-784. doi: 10.1016/j.funbio.2017.05.005 URL
[33]	Ma L J, Geiser D M, Proctor R H, et al. Fusarium pathogenomics[J]. Annual review of microbiology, 2013,67(1):399-416. doi: 10.1146/annurev-micro-092412-155650 URL
[34]	Liu T B. The F-Box protein Fbp1 is a novel virulence factor that shapes the immunogenic potential of Cryptococcus neoformans[ 中国菌物学会2018年学术年会论文汇编, 2018.
[35]	Canero D C, Roncero M I G. Influence of the chloride channel of Fusarium oxysporum on extracellular laccase activity and virulence on tomato plants[J]. Microbiology, 2008,154(5):1474-1481. doi: 10.1099/mic.0.2007/015388-0 URL
[36]	López B M S, Manuel S, et al. The velvet complex governs mycotoxin production and virulence of Fusarium oxysporum on plant and mammalian hosts[J]. Molecular Microbiology, 2013,87(1):49-65. doi: 10.1111/mmi.2013.87.issue-1 URL
[37]	Ruiz R C, Jaime Y P, Reyes J A G, et al. The Transcription Factor Con7-1 Is a Master Regulator of Morphogenesis and Virulence in Fusarium oxysporum[J]. Molecular Plant Microbe Interactions Mpmi, 2015,28(1):55-68. doi: 10.1094/MPMI-07-14-0205-R URL
[38]	van der D C, Like Fokkens L, Yang A, et al. Transcription factors encoded on core and accessory chromosomes of Fusarium oxysporum induce expression of effector genes[J]. Plos Genetics, 2016,12(11):e1006527. doi: 10.1371/journal.pgen.1006527 URL
[39]	Michielse C B, Vijk R, Reijnen L, et al. The nuclear protein sge1 of Fusarium oxysporum is required for parasitic growth[J]. Plos Pathogens, 2009,5(10):e1000637. doi: 10.1371/journal.ppat.1000637 URL
[40]	Sanchez J N, Castillo V C D, Tello V, et al. The FTF gene family regulates virulence and expression of SIX effectors in Fusarium oxysporum[J]. Molecular Plant Pathology, 2016,17(7):1224-1239.
[41]	Hou X R, An B, Wang Q N, et al. SGE1 is involved in conidiation and pathogenicity of Fusarium oxysporum f.sp. cubense[J]. Canadian Journal of Microbiology, 2018,64(5):349-357. doi: 10.1139/cjm-2017-0638 URL
[42]	Nieto F C, Pietro A D, Roncero M I G, et al. Role of the transcriptional activator XlnR of Fusarium oxysporum in regulation of xylanase genes and virulence[J]. Molecular Plant Microbe Interactions Mpmi, 2007,20(8):977-985. doi: 10.1094/MPMI-20-8-0977 URL
[43]	Ruiz G B, Roldán C R, Roncero M I G. Lipolytic system of the tomato pathogen Fusarium oxysporum f.sp.lycopersici[J]. Molecular Plant-microbe Interactions, 2013,26(9):1054-1067. doi: 10.1094/MPMI-03-13-0082-R URL
[44]	Caracuel Z, Roncero M I G, Espeso E A, et al. The pH signalling transcription factor PacC controls virulence in the plant pathogen Fusarium oxysporum[J]. Molecular Microbiology, 2003,48(3):765-779. doi: 10.1046/j.1365-2958.2003.03465.x URL
[45]	Huang W, Shang Y F, Chen P L, et al. MrpacC regulates sporulation, insect cuticle penetration and immune evasion in Metarhizium robertsii[J]. Environmental Microbiology, 2015,42(10):1462-2920.
[46]	李敏慧, 苑曼琳, 姜子德, 等. 香蕉枯萎病菌致病机理研究进展[J]. 果树学报, 2019,36(6):803-811.
[47]	Chen R, Jiang N, Jiang Q Y, et al. Exploring MicroRNA-Like small RNAs in the filamentous fungus Fusarium oxysporum[J]. Plos One, 2014,9(8):e104956. doi: 10.1371/journal.pone.0104956 URL
[48]	Quoc N B, Nakayashiki H. RNA Silencing in filamentous fungi: from basics to applications[J]. Genetic Transformation Systems in Fung, 2015,2:107-124.
[49]	Park G, Borkovich K A. Small RNA isolation and library construction for expression profiling of small RNAs from Neurospora and Fusarium using Illumina High-Throughput Deep Sequencing[J]. RNA Abundance Analysis, 2012,883:155-164.
[50]	林漪莲, 王鸿飞, 苑曼琳, 等. 香蕉枯萎病菌milRNA生物合成相关基因QDE2的功能研究[C]. 中国植物病理学会, 2018.