Detection Technology of Sedative Residues in Aquatic Products: Research Progress

doi:10.11924/j.issn.1000-6850.casb2020-0064

Abstract

Abstract:

China's fresh aquatic products market is growing rapidly, but the geographical difference between production and sales is obvious, and long-distance transportation demand is significant. The sedative can inhibit the body's metabolism and slow down the stress response of fresh aquatic products. Sedatives have been used in the transportation, breeding and surgery of aquatic products to increase the survival rate of aquatic products. However, the residual problems that may be caused by the widespread use of sedatives in aquatic products have attracted the attention of researchers at home and abroad. In this paper, the research progress of the detection technology of sedative residues in aquatic products was summarized, in order to provide reference for the follow-up research. This paper introduced the types and usage regulations of sedatives in aquatic products, and analyzed the research progress of sedatives in aquatic products in the past 10 years. The progress of sample preparation methods, HPLC detection methods, HPLC-MS/MS, and GC-MS/MS in the detection of sedative residues in aquatic products were reviewed, and the application prospects of the technologies were discussed. On the basis of the current study, more in-depth research should be carried out on the new simple and efficient sample preparation methods in the future to provide technical support for the supervision of sedatives in aquatic products.

Key words: aquatic products, sedative, detection technology, progress

CLC Number:

S912

Li Qin, Mu Shuhe, Han Gang, Liu Huan, Wu Lidong, Xu Yuyan, Li Jincheng. Detection Technology of Sedative Residues in Aquatic Products: Research Progress[J]. Chinese Agricultural Science Bulletin, 2021, 37(12): 86-91.

Figures/Tables 1

References 52

[1]	惠芸华, 蔡友琼, 于慧娟. 镇静类药物在活鱼运输中的应用研究进展[J]. 中国渔业质量与标准, 2014,4(2):39-43.
[2]	李晓芹, 朱振华, 翟纹静, 等. 渔用麻醉剂使用现状和检测技术研究进展[J]. 安徽农业科学, 2017,45(29):72-73,170.
[3]	李园园, 戴相辉. 全自动固相萃取技术同时测定水产品中7种局部麻醉剂药物残留[C]. 北京: 2014.
[4]	方晓磊, 柯常亮, 刘奇, 等. 水产品中丁香酚残留的人体健康风险分析[J]. 水产科学, 2018,37(1):140-144.
[5]	乌仁图雅, 邓宇, 荣迪, 等. 超高效液相色谱法检测土壤中硝基呋喃类药物残留[J]. 化学分析计量, 2020,29(3):21-25.
[6]	郑向华, 孙婷, 陈燕, 等. 固相萃取-液相色谱串联质谱法同时测定水产品中3种鱼用麻醉剂残留量[J]. 食品科技, 2020,45(4):333-337.
[7]	柯常亮, 刘奇, 陈洁文, 等. 气相色谱-串联质谱联用法测定水中丁香酚残留[J]. 中国渔业质量与标准, 2014,4(4):49-55.
[8]	农业部畜牧兽医局. 农业部发布动物性食品中兽药最高残留限量(续)[J]. 中国兽药杂志. 2003,25(2):5-11.
[9]	中华人民共和国农业部公告第176号[S]. 北京:中华人民共和国农业部, 2002.
[10]	中华人民共和国农业部公告第235号[S]. 北京:中华人民共和国农业部, 2002.
[11]	Li J, Zhang J, Liu Y. Optimization of solid-phase-extraction cleanup and validation of quantitative determination of eugenol in fish samples by gas chromatography-tandem mass spectrometry[J]. Analytical & Bioanalytical Chemistry. 2015,407(21):6563-6568.
[12]	Variation Instrument No. APVMA 12, Australia New Zealand Food Standards Code - Schedule 20-Maximum residue limits[S]. 2017.
[13]	FDA. Freedom of Information Summary[J]. NADA, Silver Spring, MD, 2015, 200-226.
[14]	Government of Canada, Health Canada, Health Products and Food Branch, Veterinary Drugs Directorate. List of Veterinary Drugs that are Authorized for Sale by Health Canada for Use in Food-Producing Aquatic Animals - Health Canada[S].
[15]	Government of Canada, Health Canada, Health Products and Food Branch, Veterinary Drugs Directorate. Proposal to amend the List of Maximum Residue Limits for veterinary drugs in foods (Proposed MRL 2013-1) - Health Canada Consultation Document[S].
[16]	Zahl I H, Kiessling A. Anaesthesia of farmed fish: implications for welfare[J]. Fish Physiology & Biochemistry. 2012,38(1):201-218.
[17]	李晋成, 刘欢, 吴立冬, 等. 动物体内麻醉剂残留检测技术研究进展[J]. 食品科学, 2014,35(5):251-256.
[18]	Chen Y, Guo Z, Wang X, et al. Sample preparation[J]. Journal of Chromatography A. 2008,1184(1):191-219.
[19]	Li J, Liu H, Wang C, et al. Determination of eugenol in fish and shrimp muscle tissue by stable isotope dilution assay and solid-phase extraction coupled gas chromatography-triple quadrupole mass spectrometry[J]. Analytical & Bioanalytical Chemistry, 2016,408(24):6537-6544.
[20]	Scherpenisse P, Bergwerff A A. Determination of residues of tricaine in fish using liquid chromatography tandem mass spectrometry[J]. Analytica Chimica Acta, 2007,586(1-2):407-410. pmid: 17386741
[21]	朱敏, 孙伟红, 邢丽红, 等. 液相色谱-串联质谱法测定水产品中麻醉剂MS-222残留[J]. 分析试验室, 2012(6):59-62.
[22]	高平, 黄和, 刘文侠. 固相萃取-高效液相色谱-荧光检测法测定水产品中4种丁香酚类化合物[J]. 中国食品卫生杂志, 2016,28(1):56-61.
[23]	Meinertz J R, Schreier T M, Porcher S T, et al. Depletion of eugenol residues from the skin-on fillet tissue of rainbow trout exposed to 14 C-labeled eugenol[J]. Aquaculture, 2014,430:74-78. doi: 10.1016/j.aquaculture.2014.03.050 URL
[24]	Ke C, Liu Q, Li L, et al. Simultaneous determination of eugenol, isoeugenol and methyleugenol in fish fillet using gas chromatography coupled to tandem mass spectrometry[J]. Journal of Chromatography B, 2016,1031:189-194.
[25]	赵东豪, 王强, 王旭峰. 超高效液相色谱-串联质谱法检测鱼肉和暂养水中丁香酚残留量[J].食品科学,相色谱-串联质谱法测定水产品中地西泮及其代谢物残留量[J]. 湖南农业科学, 2010(16):46-48.
[26]	钱晓东, 于慧娟, 惠芸华. 等. 高效液相色谱-串联质谱法测定水产品中地西泮及其代谢物残留量[J]. 湖南农业科学, 2010(16):46-48.
[27]	于慧娟, 王庆贺, 戚隽渊, 等. 同位素稀释液质联用法测定鱼体中氯丙嗪的残留[J]. 海洋渔业, 2011,33(1):83-90.
[28]	Huang S, Xu J, Wu J, et al. Rapid detection of five anesthetics in tilapias by in vivo solid phase microextraction coupling with gas chromatography-mass spectrometry[J]. Talanta, 2017,168(Complete):263-268. doi: 10.1016/j.talanta.2017.03.045 URL
[29]	李婷, 田佳乐, 刘洋, 等. 基于固相微萃取-气相色谱-质谱与电子鼻技术分析发酵乳中的挥发性风味物质[J]. 食品与发酵工业. 2020,46(10):233-241.
[30]	Guo T, Zhou H, Yu Y, et al. Solid-phase extraction materials based on molecularly imprinted polymers for recognition of pyrethroids[J]. Journal of Applied Polymer Science, 2020,137(31).
[31]	Ansari S, Karimi M. Novel developments and trends of analytical methods for drug analysis in biological and environmental sample by molecularly imprinted polymers[J]. Trac Trends in Analytical Chemistry, 2017,89(Complete):146-162. doi: 10.1016/j.trac.2017.02.002 URL
[32]	Qin S, Su L, Peng W, et al. Mixed templates molecularly imprinted solid-phase extraction for the detection of sulfonamides in fish farming water[J]. Journal of Applied Polymer Science, 2015,132(8).
[33]	田春霞. 分子印迹固相萃取技术在食品安全检测中的应用进展[J]. 化学试剂, 2017,39(11):1175-1178.
[34]	Hui S, Jia-Ping L, Fang C, et al. Molecularly imprinted microspheres synthesized by a simple, fast, and universal suspension polymerization for selective extraction of the topical anesthetic benzocaine in human serum and fish tissues[J]. Analytical & Bioanalytical Chemistry, 2015,407(6):1745-1752.
[35]	Seyed M D, Amin J D, Andersson L I, et al. Molecularly imprinted polymer in microextraction by packed sorbent for the simultaneous determination of local anesthetics: lidocaine, ropivacaine, mepivacaine and bupivacaine in plasma and urine samples[J]. Biomedical Chromatography Bmc, 2013,27(11):1481-1488. doi: 10.1002/bmc.v27.11 URL
[36]	Yang L, Zhao F, Zeng B. Electrochemical determination of eugenol using a three-dimensional molecularly imprinted poly (p -aminothiophenol-co- p -aminobenzoic acids) film modified electrode[J]. Electrochimica Acta, 2016,210:293-300. doi: 10.1016/j.electacta.2016.05.167 URL
[37]	Sorribes-Soriano A, Esteve-TurrillasF A, Armenta S, et al. Cocaine abuse determination by ion mobility spectrometry using molecular imprinting[J]. Journal of Chromatography A, 2017,1481(Complete):23-30.
[38]	付含, 王海翔, 陈贵堂. 分子印迹技术在食品化学污染物检测分析中的应用[J]. 食品安全质量检测学报, 2019,10(4):992-997.
[39]	Anastassiades M, Lehotay S J, Stajnbaher D, et al. Fast and easy multiresidue method employing acetonitrile extraction/partitioning and "dispersive solid-phase extraction" for the determination of pesticide residues in produce[J]. Journal of Aoac International, 2003,86(2):412-431. pmid: 12723926
[40]	Li J, Liu H, Yu M, et al. Rapid determination of tricaine mesylate residues in fish samples using modified QuEChERS and high-performance liquid chromatography-tandem mass spectrometry[J]. Analytical methods, 2014,6(22):9124-9128. doi: 10.1039/C4AY00784K URL
[41]	高平, 陈日檬, 曾丹丹, 等. 新型QuEChERS结合固相萃取-高效液相色谱法测定水产品中6种麻醉剂[J]. 分析实验室, 2018,37(1):88-92.
[42]	邹游, 邵琳智, 陈思敏, 等. QuEChERS-高效液相色谱-串联质谱法测定动物源性食品中3种镇静剂残留[J]. 色谱, 2017,35(8):801-807.
[43]	Zou N, Chen R, Qin Y, et al. Comparison of pulse glow discharge-ion mobility spectrometry and liquid chromatography with tandem mass spectrometry based on multiplug filtration cleanup for the analysis of tricaine mesylate residues in fish and water[J]. Journal of Separation Science, 2016,39(18):3638-3646. doi: 10.1002/jssc.v39.18 URL
[44]	Xie C, Li Q, Han G, et al. Stable isotope dilution assay for the accurate determination of tricaine in fish samples by HPLC-MS-MS[J]. Biomedical Chromatography, 2019,33(5):e4512. doi: 10.1002/bmc.v33.5 URL
[45]	Li J, H H, C W, et al. Stable isotope labeling-assisted GC/MS/MS method for determination of methyleugenol in food samples[J]. Journal of the Science of Food & Agriculture, 2018,98(9):3485.
[46]	李晋成, 刘欢, 韩刚. 基于磁分离的全自动QuEChERS前处理一体机:中国, CN201920262115.6[P]. 2020.3.27.
[47]	李晋成, 韩刚, 刘欢. 推杆式滤过型净化柱: 中国, CN201920261816.8[P]. 2020.05.05.
[48]	李晋成. 全自动震荡提取净化装置: 中国, CN201920478493.8[P]. 2020.03.31.
[49]	孙鹏, 高玉玲, 王金梅, 等. 漂浮固化分散液液微萃取-UHPLC-MS/MS法测定水产品中丁香酚的残留量[J]. 质谱学报, 2017,38(3):342-348.
[50]	黄武, 徐金龙, 刘建芳, 等. 高效液相色谱法检测罗非鱼中丁香酚残留量[J]. 食品安全质量检测学报, 2018,9(1):103-106.
[51]	储成群, 梁景文, 苑婷婷, 等. HPLC-MS/MS测定黄颡鱼/桂花鱼中麻醉剂MS-222残留[J]. 安徽农业科学, 2018,46(22):154-156.
[52]	陈焕, 黄和, 高平, 等. GC-MS法同时测定水产品中6种丁香酚类麻醉剂的残留量[J]. 食品工业, 2017,38(7):305-309.

镇静剂种类	允许使用的国家及地区	休药期及最大残留限量(MRL)规定	参考文献
丁香酚	日本、台湾地区	鱼类休药期7天,甲壳类休药期10天,MRL值为50 ng/mL	[11]
异丁香酚（trans-异丁香酚和cis-异丁香酚）	澳大利亚、新西兰	MRL值为100 mg/kg（trans-异丁香酚和cis-异丁香酚的总量）	[12]
MS-222	美国、加拿大	美国FDA规定休药期21天,加拿大规定休药期7天,MRL值为1 μg/mL	[13-15]
MS-222、苯佐卡因	挪威	休药期21天	[16]

镇静剂种类	允许使用的国家及地区	休药期及最大残留限量(MRL)规定	参考文献
丁香酚	日本、台湾地区	鱼类休药期7天,甲壳类休药期10天,MRL值为50 ng/mL	[11]
异丁香酚（trans-异丁香酚和cis-异丁香酚）	澳大利亚、新西兰	MRL值为100 mg/kg（trans-异丁香酚和cis-异丁香酚的总量）	[12]
MS-222	美国、加拿大	美国FDA规定休药期21天,加拿大规定休药期7天,MRL值为1 μg/mL	[13-15]
MS-222、苯佐卡因	挪威	休药期21天	[16]