Anthracnose Disease on Prunus salicina ‘Banbianhong’: Investigation and Pathogen Identification

doi:10.11924/j.issn.1000-6850.casb2020-0328

Abstract

Abstract:

The aim is to investigate the anthracnose disease and the pathogen species in Prunus salicina ‘Banbianhong’ and provide a basis to prevent and control the disease. In this study, six P. salicina ‘Banbianhong’ orchards were taken as research objects in two major production areas, Huiyi and Xintan Town in Suijiang County, Yunnan Province, anthracnose disease was investigated and pathogenic fungi were isolated and stored. The pathogen species were identified and analyzed according to the spore morphology and ITS sequences of Colletotrichum. Disease investigation showed that the disease severity of anthracnose in Xintan was higher than that in Huiyi. A total of 15 single spore isolates were obtained, and the morphology of colonies on every strain plate had weak difference. Four kinds of conidia were obtained and all of them were pathogenic by Tie-back experiment. rDNA ITS sequence phylogenetic analysis showed that the pathogen of anthracnose disease in P. salicina ‘Banbianhong’ were Colletotrichum gloeosporioides species complex and C. siamense species complex. This study investigated the damage status of anthracnose disease in P. salicina ‘Banbianhong’, got the single spore isolates, and determined the complex groups of the pathogen of anthracnose disease in P. salicina ‘Banbianhong’.

Key words: Prunus salicina ‘Banbianhong’, anthracnose, Colletotrichum spp., morphological identification, ITS

CLC Number:

S463.65

Wang Qun, Bi Yunqing, He Chengxing, Guo Zhixiang, Zhong Dewei, Li Junhong, Chen Qianzhao, You Jihua, Huang Zhenghui, Li Jinbin. Anthracnose Disease on Prunus salicina ‘Banbianhong’: Investigation and Pathogen Identification[J]. Chinese Agricultural Science Bulletin, 2021, 37(15): 128-136.

Figures/Tables 6

References 45

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果园	海拔/m	发病率/%	病情指数
会仪镇黄坪村1	380~500	0	0.0
会仪镇黄坪村2	500~700	30	31.1
会仪镇黄坪村3	700~900	30	30.0
合计		20	20.4
新滩镇鲢鱼10队	380~500	40	41.1
新滩镇鲢鱼12队	500~700	70	70.0
新滩镇鲢鱼17队	700~900	40	63.3
合计		50	78.5

果园	海拔/m	发病率/%	病情指数
会仪镇黄坪村1	380~500	0	0.0
会仪镇黄坪村2	500~700	30	31.1
会仪镇黄坪村3	700~900	30	30.0
合计		20	20.4
新滩镇鲢鱼10队	380~500	40	41.1
新滩镇鲢鱼12队	500~700	70	70.0
新滩镇鲢鱼17队	700~900	40	63.3
合计		50	78.5

菌株	长度/μm	宽度/μm	长宽比
18-4-C2	18.35±1.70(15.9~21.6) B	4.72±0.64(4.0~6.1) A	3.94±0.58(3.0~4.8) B
18-8-C1	13.35±1.14(11.9~15.1) C	4.00±0.55(3.3~4.8) B	3.40±0.62(2.9~4.6) B
18-8-D1	21.94±2.25(18.2~26.1) A	2.62±0.52(2.1~3.5) C	8.65±1.91(6.7~12.2) A
18-5-B1	14.65±1.94(12.8~19.1) C	4.35±0.53(3.6~5.2) AB	3.44±0.82(2.5~5.4) B

菌株	长度/μm	宽度/μm	长宽比
18-4-C2	18.35±1.70(15.9~21.6) B	4.72±0.64(4.0~6.1) A	3.94±0.58(3.0~4.8) B
18-8-C1	13.35±1.14(11.9~15.1) C	4.00±0.55(3.3~4.8) B	3.40±0.62(2.9~4.6) B
18-8-D1	21.94±2.25(18.2~26.1) A	2.62±0.52(2.1~3.5) C	8.65±1.91(6.7~12.2) A
18-5-B1	14.65±1.94(12.8~19.1) C	4.35±0.53(3.6~5.2) AB	3.44±0.82(2.5~5.4) B