Abstract: Effects of 6-benzylaminopurine concentration, cultivar, explant size and AgNO3 concentration on direct shoot induction were studied by sing cotyledonary nodes as explants of cucumber (Cucumis sativus L.). Results showed different cultivar showed significant difference in the frequency of inducing shoots and number of shoots per explant and showed no difference in shoot length. 4.0mg/L 6-benzylaminopurine was the optimal concentration of shoots quantity inducing which frequency of inducing shoots and number of shoots per explant were 100% and 11.1 respectively. 0.5mg/L 6-benzylaminopurine was the optimal concentration of shoots length inducing which average length of shoots was 12.0mm. Explant size had significantly effects on direct shoots quantity and length induction. When complete cotyledon used as explant, frequency of inducing shoots, number of shoots per explant and average length of shoots could approach to 100%, 6.7 and 17.1mm respectively. 2.0mg/L silver nitrate could significantly increase shoot quantity induction, both frequency of inducing shoots and number of shoots per explant could be as high as 97.2% and 4.2 respectively. But silver nitrate also could restrain shoots elongation.