Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2021, Vol. 37 ›› Issue (36): 19-23.doi: 10.11924/j.issn.1000-6850.casb2021-0212

Special Issue: 玉米

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Preventing Transgene Drift of Maize: The Test Strip Rapid Detection Method

CUI Guimei1(), LI Xiaobo1, MA Yue1, HAO Yaoshan2, ZHANG Huanhuan2, SUN Yi2()   

  1. 1Shanxi Dafeng Seeds Company Ltd., Taiyuan 030031
    2College of Life Sciences, Shanxi Agricultural University, Taiyuan 030031
  • Received:2021-03-05 Revised:2021-07-08 Online:2021-12-25 Published:2022-02-15
  • Contact: SUN Yi E-mail:dfzycgm@163.com;sunyi692003@163.com

Abstract:

The purpose of this study is to detect transgenic plants from conventional maize breeding materials at seedling stage by using test strips, and to prevent unexpected transgene drift. Transgenic and non-transgenic maize lines were used as materials in this study. Based on the experiences of screening transgenic maize materials in the past, test strip rapid detection method was obtained. The authors introduced the supplies required for examination and sampling methods, and discussed the sample quantity, operation steps and precautions in detail. The authors also demonstrated and analyzed the processes and results of two typical detection cases. The operation steps of this method are simple and easy to learn, and the method is suitable for professional and non-professional breeders or inspectors to use in self-examination or inspection of field materials. The test strip rapid detection method can effectively screen and prevent the transgene drift caused by unexpected mixing of pollen and seeds.

Key words: maize, transgene drift, pollen, the test strip rapid detection

CLC Number: