Mapping of Plant Height QTL Using NILs Derived from Residual Heterozygous Lines in Rice

doi:10.11924/j.issn.1000-6850.casb2021-0510

Abstract

Abstract:

To explore a novel and valuable gene for plant height in rice breeding, a residual heterozygous line (RHL) RHL1030 (F₁₀) derived from a cross between ‘Katy’ and ‘Xiang743’, with a heterozygous interval of RM11383-RM1198 on chromosome 1 was used as the genetic material. Genetic analysis of RHL1030-derived population showed that a QTL for plant height was identified in the interval, the additive-effect QTL came from ‘Xiang743’. Two individual plants respectively with heterozygous intervals of RM3411-RM11782 and RM6703-RM1198 were selected from the RHL1030-derived population on the basis of SSR genotypes, and self-pollinated to develop two F₂ populations. The QTL was validated and further delimited in the interval of RM6703-RM1198. Three individual plants with heterozygous intervals of RM6703-RM8085 and one individual plant with a heterozygous interval of RM5389-RM1198 were selected from the population derived from a RHL with a heterozygous interval of RM6703-RM1198, and self-pollinated to develop four resultant F₂ populations. Forty maternal homozygotes, paternal homozygotes and heterozygotes respectively were selected from the four F₂ populations to develop near isogenic lines for ANOVA analysis. The QTL was finally delimited in the interval of RM11782-RM5389, and the corresponding physical position was from 34.17 Mb to 35.73 Mb. This study identifies a novel QTL for plant height, and provides a resource for improving plant architecture in rice.

Key words: residual heterozygous line (RHL), near isogenic line (NIL), plant height, rice (Oryza sativa L.), quantitative trait locus (QTL)

CLC Number:

S184

LUO Xianfu, LIU Wenqiang, PAN Xiaowu, DONG Zheng, LIU Sanxiong, LIU Licheng, YANG Biaoren, SHENG Xinnian, LI Xiaoxiang. Mapping of Plant Height QTL Using NILs Derived from Residual Heterozygous Lines in Rice[J]. Chinese Agricultural Science Bulletin, 2022, 38(9): 1-5.

Figures/Tables 3

References 20

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群体	杂合区间	基因型值¹			F	P	加性效应	显性效应	R²/%²
XK2-5	RM6703-RM8085	158.3	126.6	146.8	50.7	<0.000001	15.9	4.35	63.1
XK10-7	RM6703-RM8085	163.6	127.5	152.2	15.9	<0.000001	18.1	6.65	69.2
XK13-1	RM6703-RM8085	168.9	135.4	156.8	41.2	<0.000001	16.8	4.65	74.1
XK5-4	RM5389-RM1198	120.3	118.6	117.3	1.3	0.26

群体	杂合区间	基因型值¹			F	P	加性效应	显性效应	R²/%²
XK2-5	RM6703-RM8085	158.3	126.6	146.8	50.7	<0.000001	15.9	4.35	63.1
XK10-7	RM6703-RM8085	163.6	127.5	152.2	15.9	<0.000001	18.1	6.65	69.2
XK13-1	RM6703-RM8085	168.9	135.4	156.8	41.2	<0.000001	16.8	4.65	74.1
XK5-4	RM5389-RM1198	120.3	118.6	117.3	1.3	0.26