Abstract: By using pre-harvest sprouting of Brassica napus lines 780、943、890、11-5、20-1 as material in the field condition, the seeds were divided into three groups of which including full germinating seeds, natural seeds, no germinating seeds, and the main qualities were tested through Infrared Spectroscopy; The effects of four kinds of seeds in terms of sprout length and seed length on 1000 seeds weights were investigated. The results show that seeds oil and erucic acid content were decreased, protein content was increased, and glucosinolate content had no regular change. 1000 seeds weight were decreased by 5.52%~22.17%.