Antifungal and Control Effect of Bacillus mojavensis on Pear Valsa Canker

doi:10.11924/j.issn.1000-6850.casb2021-1050

Abstract

Abstract:

The purpose of this study is to explore the antifungal effect and mechanism of Bacillus mojavensis on Valsa ceratosperma, and to provide a basis for biological control of pear valsa canker. The control effect of Bacillus mojavensis on pear valsa canker was studied using plate confrontation, preliminary study on biological control mechanism, and in vitro branch methods. The results showed that Bacillus mojavensis significantly inhibited the growth of V. ceratosperma, and the inhibition rate of hypha reached 68%. Bacillus mojavensis produced protease, amylase, cellulase and siderophore, and its sterile fermentation filtrate could inhibit the growth of V. ceratosperma hypha, while its volatile substances could inhibit the growth of hypha with a rate of 22.6%. The protective control efficacy of Bacillus mojavensis was 41.7%, and the therapeutic control efficacy was 44.6%. Bacillus mojavensis has obvious inhibitory effect on V. ceratosperma, and it has certain biocontrol potential on pear valsa canker.

Key words: Bacillus mojavensis, pear valsa canker, Valsa ceratosperma, biological control, control effect

CLC Number:

S432.4

LIU Long, RONG Hua, ZHENG Tongtong, MA Junjie, GUO Qingyuan. Antifungal and Control Effect of Bacillus mojavensis on Pear Valsa Canker[J]. Chinese Agricultural Science Bulletin, 2022, 38(18): 140-146.

Figures/Tables 10

References 25

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滤液浓度(v/v)	处理菌落平均直径/cm	对照菌落平均直径/cm	生长抑制率/%
0.1	5±0.31a	7.22±0.33a	33.5a
0.15	4.15±0.5b	7.03±0.31a	44.8b
0.25	2.13±0.52c	6.47±0.43a	73.9c
0.3	1.72±0.37c	5.52±0.62b	77.2c
0.4	0d	4.23±0.73c	100d

滤液浓度(v/v)	处理菌落平均直径/cm	对照菌落平均直径/cm	生长抑制率/%
0.1	5±0.31a	7.22±0.33a	33.5a
0.15	4.15±0.5b	7.03±0.31a	44.8b
0.25	2.13±0.52c	6.47±0.43a	73.9c
0.3	1.72±0.37c	5.52±0.62b	77.2c
0.4	0d	4.23±0.73c	100d

处理	莫海威芽孢杆菌接种7 d,腐烂病菌接种4 d			莫海威芽孢杆菌接种10 d,腐烂病菌接种7 d
处理	发病率	病斑面积/cm²	防效/%	病斑面积/cm²	防效/%
预防	100%	0.29±0.03	52	3.49±0.89	41.7
CK	100%	0.6±0.04	—	5.96±1.04	—

处理	莫海威芽孢杆菌接种7 d,腐烂病菌接种4 d			莫海威芽孢杆菌接种10 d,腐烂病菌接种7 d
处理	发病率	病斑面积/cm²	防效/%	病斑面积/cm²	防效/%
预防	100%	0.29±0.03	52	3.49±0.89	41.7
CK	100%	0.6±0.04	—	5.96±1.04	—

处理	莫海威芽孢杆菌接种3 d		莫海威芽孢杆菌接种7 d
处理	病斑面积/cm²	防效/%	病斑面积/cm²	防效/%
治疗	1.51±0.36	49	2.91±0.73	44.6
CK	2.96±0.4	—	5.25±0.97	—