Ultrasonic Extraction Process of Total Saponins from Leaves of Solanum torvum Swartz.

doi:10.11924/j.issn.1000-6850.casb2021-1055

Abstract

Abstract:

In order to improve the extraction rate of total saponins from leaves of Solanum torvum Swartz., the study optimized the extraction process. Taking the extraction rate of total saponins as the evaluation index, we studied the effects of ethanol concentration, solid-liquid ratio, extraction time and extraction temperature on the extraction rate by single factor experiment. On the basis of single factor experiment, response surface methodology was carried out to optimize the ultrasonic extraction process of total saponins from leaves of Solanum torvum Swartz.. The results showed that the optimum technological condition was ethanol concentration of 80.30%, solid-liquid ratio of 1:15.06 (g/mL), extraction time of 95.56 min and extraction temperature of 62.29℃. Under this condition, the theoretical extraction rate of total saponins from leaves of Solanum torvum Swartz. was 38.82%, and the response surface model had good fitting, good prediction and reliable operation. The ultrasonic extraction process of total saponins from leaves of Solanum torvum Swartz. optimized by response surface methodology is stable and feasible.

Key words: Solanum torvum Swartz., leaf, total saponins, ultrasonic extraction, response surface methodology

CLC Number:

WANG Wenxuan, LI Yunpeng, XU Hening, ZHAO Xin, MA Rou, ZHANG Lina, ZHANG Yingying. Ultrasonic Extraction Process of Total Saponins from Leaves of Solanum torvum Swartz.[J]. Chinese Agricultural Science Bulletin, 2022, 38(30): 118-125.

Figures/Tables 13

References 25

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编码值	因素
编码值	A乙醇浓度/ %	B料液比/ (g/mL)	C提取时间/ min	D提取温度/ ℃
-1	75	1:10	60	50
0	80	1:15	90	60
1	85	1:20	120	70

编码值	因素
编码值	A乙醇浓度/ %	B料液比/ (g/mL)	C提取时间/ min	D提取温度/ ℃
-1	75	1:10	60	50
0	80	1:15	90	60
1	85	1:20	120	70

试验号	A乙醇浓度/%	B料液比/ (g/mL)	C提取时间/min	D提取温度/℃	皂苷提取率/%	试验号	A乙醇浓度/%	B料液比/ (g/mL)	C提取时间/min	D提取温度/℃	皂苷提取率/%
1	80	10	120	60	32.87	16	80	20	90	50	29.64
2	80	15	90	60	37.76	17	85	15	120	60	33.98
3	75	20	90	60	33.24	18	85	15	90	70	32.37
4	75	15	60	60	27.28	19	85	20	90	60	33.35
5	80	15	60	50	21.01	20	80	20	90	70	32.41
6	80	10	90	50	26.52	21	80	15	90	60	37.9
7	80	20	120	60	33.55	22	85	10	90	60	33.24
8	75	15	90	70	31.25	23	80	15	90	60	38.74
9	80	15	120	70	31.47	24	80	15	90	60	38.52
10	75	15	90	50	25.73	25	85	15	90	50	26.42
11	80	15	60	70	26.5	26	80	10	60	60	28.32
12	80	10	90	70	32.37	27	75	15	120	60	33.09
13	80	20	60	60	30.83	28	75	10	90	60	32.88
14	85	15	60	60	27.03	29	80	15	120	50	27.68
15	80	15	90	60	38.52

试验号	A乙醇浓度/%	B料液比/ (g/mL)	C提取时间/min	D提取温度/℃	皂苷提取率/%	试验号	A乙醇浓度/%	B料液比/ (g/mL)	C提取时间/min	D提取温度/℃	皂苷提取率/%
1	80	10	120	60	32.87	16	80	20	90	50	29.64
2	80	15	90	60	37.76	17	85	15	120	60	33.98
3	75	20	90	60	33.24	18	85	15	90	70	32.37
4	75	15	60	60	27.28	19	85	20	90	60	33.35
5	80	15	60	50	21.01	20	80	20	90	70	32.41
6	80	10	90	50	26.52	21	80	15	90	60	37.9
7	80	20	120	60	33.55	22	85	10	90	60	33.24
8	75	15	90	70	31.25	23	80	15	90	60	38.74
9	80	15	120	70	31.47	24	80	15	90	60	38.52
10	75	15	90	50	25.73	25	85	15	90	50	26.42
11	80	15	60	70	26.5	26	80	10	60	60	28.32
12	80	10	90	70	32.37	27	75	15	120	60	33.09
13	80	20	60	60	30.83	28	75	10	90	60	32.88
14	85	15	60	60	27.03	29	80	15	120	50	27.68
15	80	15	90	60	38.52

方差来源	平方和	自由度	均方	F值	P值	显著性
Model	536.87	14	38.35	66.18	<0.0001	**
A乙醇浓度	0.71	1	0.71	1.23	0.2868	-
B料液比	3.87	1	3.88	6.69	0.0215	*
C提取时间	83.58	1	83.58	144.25	<0.0001	**
D提取温度	71.88	1	71.88	0.027	<0.0001	**
方差来源	平方和	自由度	均方	F值	P值	显著性
AB	0.016	1	0.016	0.56	0.8719	-
AC	0.32	1	0.32	0.080	0.4664	-
AD	0.046	1	0.046	1.44	0.7817	-
BC	0.84	1	0.84	4.09	0.2493	-
BD	2.37	1	2.37	1.25	0.0626	-
CD	0.72	1	0.72	103.31	0.2829	-
A²	59.86	1	59.85	39.05	<0.0001	**
B²	22.63	1	22.63	287.65	<0.0001	**
C²	166.67	1	166.67	-	<0.0001	**
D²	261.47	1	0.58	3.98	<0.0001	**
残差	8.11	14	0.74	-	-	-
失拟项	7.37	10	0.19	-	0.0977	-
纯误差	0.74	4	-	-	-	-
总回归	544.98	28	-	-	-	-