Endophytic Bacteria from Maize Seed with Growth-promoting Potential: Isolation, Identification and Optimization of Fermentation Conditions

doi:10.11924/j.issn.1000-6850.casb2022-0591

Abstract

Abstract:

The aim of this study is to isolate and identify endophytic bacteria with growth-promoting function from maize seeds, and to provide strain resources and technical support for the research and development of bacterial fertilizer. In this experiment, maize seeds were used as the test materials, and endophytic bacteria were isolated and purified by plate coating and three-zone marking method. Drip irrigation test was used to screen growth-promoting strains. The fermentation conditions were optimized by single factor test and orthogonal test. The promoting effect of the strains was verified by pot drip irrigation test. Bacillus subtilis was isolated and identified as SYL4, which had a relatively strong growth-promoting effect. The optimal culture condition for SYL4 was: peptone 1.5%, corn flour 0.5%, CaCO₃ 0.25 %, inoculation volume 5%, rotation speed 200 r/min, temperature 34℃, and incubation time 48 h. Drip irrigation of maize with fermentation broth increased root length and leaf length by 12.86% and 9.4%, root weight and leaf weight by 6.52% and 6%, respectively. In this study, an endophytic bacterium SYL4 with growth-promoting potential was isolated. The optimized fermentation condition increased the activity of peroxidase (POD) in maize seedlings and effectively promoted the growth of maize seedlings.

Key words: maize, endophytic bacteria, enzyme activity, plant growth-promoting, optimization of fermentation conditions

YANG Tai, YU Huiying, LU Liming, FU Haiyan, LI Guoliang, DAI Wenlong, LIU Chunguang, YANG Fengshan, MA Yukun. Endophytic Bacteria from Maize Seed with Growth-promoting Potential: Isolation, Identification and Optimization of Fermentation Conditions[J]. Chinese Agricultural Science Bulletin, 2023, 39(9): 16-23.

Figures/Tables 7

References 33

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菌株号	根重/g	根长/cm	叶重/g	叶长/cm
CK	0.34±0.03c	6.31±0.03b	0.33±0.01c	8.13±0.04c
SYL1	0.35±0.03c	6.51±0.03b	0.32±0.02c	8.31±0.05c
SYL2	0.40±0.02b	7.33±0.03ab	0.39±0.03b	10.35±0.03b
SYL3	0.34±0.02c	6.48±0.03b	0.33±0.02c	8.22±0.05c
SYL4	0.47±0.03a	8.26±0.03a	0.50±0.02a	12.92±0.03a
SYL5	0.41±0.02b	7.72±0.02a	0.43±0.02b	11.71±0.05a
SYL6	0.35±0.02c	6.25±0.03b	0.31±0.02c	8.36±0.05c

菌株号	根重/g	根长/cm	叶重/g	叶长/cm
CK	0.34±0.03c	6.31±0.03b	0.33±0.01c	8.13±0.04c
SYL1	0.35±0.03c	6.51±0.03b	0.32±0.02c	8.31±0.05c
SYL2	0.40±0.02b	7.33±0.03ab	0.39±0.03b	10.35±0.03b
SYL3	0.34±0.02c	6.48±0.03b	0.33±0.02c	8.22±0.05c
SYL4	0.47±0.03a	8.26±0.03a	0.50±0.02a	12.92±0.03a
SYL5	0.41±0.02b	7.72±0.02a	0.43±0.02b	11.71±0.05a
SYL6	0.35±0.02c	6.25±0.03b	0.31±0.02c	8.36±0.05c

生理生化反应	结果	生理生化反应	结果
V-P 试验	+	明胶液化试验	+
过氧化氢酶试验	+	苯丙氨酸脱氨酶	-
吲哚试验	-	柠檬酸盐试验	+
淀粉水解试验	+	葡萄糖	+
硝酸盐还原试验	+	甘露醇发酵	-

生理生化反应	结果	生理生化反应	结果
V-P 试验	+	明胶液化试验	+
过氧化氢酶试验	+	苯丙氨酸脱氨酶	-
吲哚试验	-	柠檬酸盐试验	+
淀粉水解试验	+	葡萄糖	+
硝酸盐还原试验	+	甘露醇发酵	-