SSR Markers of Maize and Sorghum: The Transferability in Adlay

doi:10.11924/j.issn.1000-6850.casb2022-0152

Abstract

Abstract:

In order to select available molecular makers for adlay germplasm and genetics research, the transferability and polymorphism of SSR markers from maize and sorghum were tested in adlay. 11 adlay germplasms were selected as materials, and then 364 SSR primers from maize and sorghum were screened by SSR-PCR and capillary electrophoresis methods. The results showed that 163 out of the 364 markers were successfully amplified in adlay, among which 44 markers showed well polymorphism. The percentage of transferability and polymorphism of SSR markers from sorghum were 58.02% and 30.85%, respectively, while those from maize were 23.45% and 26.47%, respectively. Sorghum SSR markers exhibited better transferability and polymorphism in adlay. A total of 110 alleles were amplified using the 44 SSR primers in the 11 adlay germplasms, with a number of bands ranging from 1 to 5 (an average of 2.5). PIC ranged from 0.15 to 0.79 and 15 SSR primers had PIC greater than 0.5, accounting for 34.09% of the total polymorphic SSR primers. Therefore, the transferable markers could provide useful genetic markers for germplasm diversity and molecular genetics research in adlay, and they could also be a powerful tool for comparative genomics among sorghum, maize and adlay.

Key words: maize, sorghum, adlay, SSR markers, transferability

FU Yuhua, MENG Qiuyi, SHANG Kun, LI Xiushi, LIU Fanzhi, LI Xiangdong. SSR Markers of Maize and Sorghum: The Transferability in Adlay[J]. Chinese Agricultural Science Bulletin, 2023, 39(5): 33-38.

Figures/Tables 4

References 33

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引物编号	引物名称	片段大小/bp	多态性位点	PIC	引物编号	引物名称	片段大小/bp	多态性位点	PIC
sb008	Txp80	272~290	4	0.612	sb118	xtxp210	200~208	2	0.298
sb016	SB5407	150~152	2	0.165	sb120	xcup47	187~206	3	0.512
sb019	SB5O14	232~257	2	0.298	sb128	xcup48	117~124	3	0.760
sb022	Txp481	212~220	3	0.512	sb132	xtxp258	121~136	3	0.314
sb029	Txp130	208~296	3	0.165	sb140	xcup66	257~318	3	0.512
sb065	Xcup02	401~407	2	0.165	sb149	xcup19	112~118	2	0.397
sb069	Xcup61	254~274	2	0.314	sb160	xtxp115	204~212	2	0.298
sb070	Xcup62	150~159	3	0.430	zm019	umc1432y6	206~242	3	0.165
sb071	Xcup63	102~158	4	0.612	zm021	umc1147y4	168~197	4	0.595
sb075	Xtxp114	109~164	3	0.298	zm028	umc1999y3	208~227	3	0.512
sb087	xcup65	222	1	0.165	zm043	bnlg1484	142~177	3	0.612
sb088	xcup32	182~201	2	0.512	zm044	bnlg1866	112~131	2	0.397
sb089	xcup34	136~166	4	0.314	zm084	phi024	137~183	2	0.165
sb091	xcup40	235~263	3	0.314	zm105	bnlg1305	161~200	2	0.165
sb095	xcup74	221~228	2	0.562	zm135	phi115	295~306	2	0.645
sb096	xcup58	176~200	3	0.612	zm139	phi024	183~191	2	0.298
sb098	xcup01	126~132	2	0.496	zs008	phi308707	121~128	2	0.165
sb102	xcup06	340-347	2	0.397	zs017	phi053	250~257	2	0.298
sb103	xcup27	241~303	5	0.793	zs033	umc1792	261	1	0.165
sb108	xgap36	176~196	2	0.165	zs037	umc1805	165~175	2	0.512
sb113	xcup12	102~116	3	0.165	zs042	phi114	151~157	2	0.397
sb115	xcup17	255	1	0.496	zs050	bnlg1724	123~128	2	0.298

引物编号	引物名称	片段大小/bp	多态性位点	PIC	引物编号	引物名称	片段大小/bp	多态性位点	PIC
sb008	Txp80	272~290	4	0.612	sb118	xtxp210	200~208	2	0.298
sb016	SB5407	150~152	2	0.165	sb120	xcup47	187~206	3	0.512
sb019	SB5O14	232~257	2	0.298	sb128	xcup48	117~124	3	0.760
sb022	Txp481	212~220	3	0.512	sb132	xtxp258	121~136	3	0.314
sb029	Txp130	208~296	3	0.165	sb140	xcup66	257~318	3	0.512
sb065	Xcup02	401~407	2	0.165	sb149	xcup19	112~118	2	0.397
sb069	Xcup61	254~274	2	0.314	sb160	xtxp115	204~212	2	0.298
sb070	Xcup62	150~159	3	0.430	zm019	umc1432y6	206~242	3	0.165
sb071	Xcup63	102~158	4	0.612	zm021	umc1147y4	168~197	4	0.595
sb075	Xtxp114	109~164	3	0.298	zm028	umc1999y3	208~227	3	0.512
sb087	xcup65	222	1	0.165	zm043	bnlg1484	142~177	3	0.612
sb088	xcup32	182~201	2	0.512	zm044	bnlg1866	112~131	2	0.397
sb089	xcup34	136~166	4	0.314	zm084	phi024	137~183	2	0.165
sb091	xcup40	235~263	3	0.314	zm105	bnlg1305	161~200	2	0.165
sb095	xcup74	221~228	2	0.562	zm135	phi115	295~306	2	0.645
sb096	xcup58	176~200	3	0.612	zm139	phi024	183~191	2	0.298
sb098	xcup01	126~132	2	0.496	zs008	phi308707	121~128	2	0.165
sb102	xcup06	340-347	2	0.397	zs017	phi053	250~257	2	0.298
sb103	xcup27	241~303	5	0.793	zs033	umc1792	261	1	0.165
sb108	xgap36	176~196	2	0.165	zs037	umc1805	165~175	2	0.512
sb113	xcup12	102~116	3	0.165	zs042	phi114	151~157	2	0.397
sb115	xcup17	255	1	0.496	zs050	bnlg1724	123~128	2	0.298