Anthocyanin in Lavender of Two Flower Colors: Analysis of Compositions

doi:10.11924/j.issn.1000-6850.casb2022-0630

Abstract

Abstract:

The aim is to clarify the composition and content differences of anthocyanin between two kinds of lavender varying in flower color, and to explore the relevance between lavender flower color and anthocyanin. The flower organs of ‘Xinxun No. 2’ (blue flower) and ‘YXA-05’ (white flower) were used as research materials. The composition and content of anthocyanins in flower organs of the two lavender varieties were determined by using the combination technology of ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF/MS). The result showed that the total anthocyanin content in ‘Xinxun No. 2’ and ‘YXA-05’ was 42.24 μg/g and 35.96 μg/g respectively, and both two varieties contained 6 types of anthocyanin, including cyanidin, peonidin, delphinidin, petunidin, malyidin and pelargonidin. There were 39 types of anthocyanin in ‘Xinxun No. 2’, and 37 types of anthocyanin in ‘YXA-05’. ‘Xinxun No. 2’ contained 6 types of anthocyanin that were not found in ‘YXA-05’, while ‘YXA-05’ contained 4 types of anthocyanin that were not found in ‘Xinxun No. 2’. It was indicated that in these two lavender varieties, anthocyanin was rich in types and differed in composition and content. The flower color of corolla was related to the composition and content of its anthocyanin.

Key words: ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry, ‘Lavender Xinxun No. 2’, anthocyanin, delphinidin, petunidin

LAI Ganggang, LI Min, JIANG Xinming, XU Panpan, LI Ziyu, GUO Danli. Anthocyanin in Lavender of Two Flower Colors: Analysis of Compositions[J]. Chinese Agricultural Science Bulletin, 2023, 39(15): 39-45.

Figures/Tables 5

References 26

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编号		苷元类型	化合物名称		含量/(μg/g)
编号		苷元类型	化合物名称		蓝花	白花
1		Cyanidin [矢车菊素]	Cyanidin-3,5-O-diglucoside[矢车菊素-3,5-O-二葡萄糖苷]		0.4463±0.0863	0.2100±0.0407
2			Cyanidin-3-O-glucoside[矢车菊素-3-O-葡萄糖苷]		0.0671±0.0013	0.0411±0.0021
3			Cyanidin-3-O-5-O-(6-O-coumaroyl)-diglucoside [矢车菊素-3-O-5-O-(6-O-对香豆酰)-二葡萄糖苷]		14.7591±3.364 9	7.6792±1.1506
4			Cyanidin-3,5,3'-O-triglucoside[矢车菊素-3,5,3'-O-三葡萄糖]		--	--
5			Cyanidin-3-O-arabinoside[矢车菊素-3-O-阿拉伯糖苷]		--	0.0137±0.0407
6			Cyanidin-3-O-sophoroside[矢车菊素-3-O-槐糖苷]		0.0143±0.0098	0.0143±0.0002
7		Delphinidin [飞燕草素]	Delphinidin-3-O-(6-O-malonyl)-glucoside-3'-glucoside [飞燕草素-3-O-(6-O-丙二酰基)-葡萄糖苷-3'-葡萄糖苷]		0.0522±0.0045	0.0177±0.0031
8			Delphinidin-3-O-glucoside[飞燕草素-3-O-葡萄糖苷]		0.0300±0.0029	0.0369±0.0041
9			Delphinidin-3,5-O-diglucoside[飞燕草素-3,5-O-二葡萄糖苷]		0.0215±0.0100	0.0152±0.0009
10			Delphinidin-3-O-(6-O-malonyl-beta-D-glucoside) [飞燕草素-3-O-(6-O-丙二酰-β-D-葡萄糖苷)]		0.2333±0.0255	0.0722±0.0067
11			Delphinidin-3-O-5-O-(6-O-coumaroyl)-diglucoside [飞燕草素-3-O-5-O-(6-O-对香豆酰)-二葡萄糖苷]		0.2394±0.0976	0.2460±0.0271
12			Delphinidin-3-O-galactoside[飞燕草素-3-O-半乳糖苷]		0.0519±0.0014	0.0254±0.0023
13			Delphinidin-3-O-rutinoside[飞燕草素-3-O-芸香糖苷]		0.0032±0.0006	0.0002±0.0002
14			Delphinidin-3-O-sophoroside[飞燕草素-3-O-槐糖苷]		0.1639±0.0064	0.0832±0.0012
15			Delphinidin[飞燕草素]		0.0029±0.0018	--
16		Malvidin [锦葵色素]	Malvidin-3,5-O-diglucoside[锦葵色素-3,5-O-二葡萄糖苷]		0.0561±0.0032	0.0244±0.0021
17			Malvidin-3-O-glucoside[锦葵色素-3-O-葡萄糖苷]		0.0334±0.0006	0.0174±0.0011
18			Malvidin-3-O-(6'-acetylglucoside)-5-glucoside [锦葵色素-3-O-(6'-乙酰基葡萄糖苷)-5-葡萄糖苷]		0.0067±0.0009	0.0041±0.0006
19			Malvidin-3-O-(6-O-p-coumaroyl)-glucoside[锦葵色素-3-(6-O-p-对香豆酰)-葡萄糖苷]		0.1877±0.0102	0.1131±0.0076
20			Malvidin-3-O-5-O-(6-O-coumaroyl)-diglucoside [锦葵色素-3-O-5-O-(6-O-对香豆酰)-二葡萄糖苷]		1.0690±0.0663	0.7004±0.0222
21			Malvidin-3-O-sophoroside[锦葵色素-3-O-槐糖苷]		0.0049±0.0012	0.0016±0.0006
22			Malvidin-3-O-(6-O-malonyl-beta-D-glucoside)[锦葵色素-3-O-6-O-丙二酰-β-D-葡萄糖苷]		0.0125±0.0017	--
23	Pelargonidin [天竺葵素]			Pelargonidin-3,5-O-diglucoside[天竺葵素-3,5-O-二葡萄糖苷]	0.0302±0.0080	0.0290±0.0162
24				Pelargonidin-3-O-(6-O-p-coumaroyl)-glucoside[天竺葵素-3-(6-O-p-对香豆酰)-葡萄糖苷]	1.1413±0.7978	1.6203±0.3566
25				Pelargonidin-3-O-glucoside[天竺葵素-3-O-葡萄糖苷]	2.5678±0.0492	3.1926±0.0845
26				Pelargonidin-3-O-sophoroside[天竺葵素-3-O-槐糖苷]	0.0119±0.0013	0.0191±0.0022
27				Pelargonidin-3-O-sophoroside-5-O-(malonyl)-glucoside [天竺葵素-3-O-槐糖苷-5-O-(丙二酰基)-葡萄糖苷]	0.0027±0.0006	0.0034±0.0002
28				Pelargonidin-3-O-(6-O-malonyl-beta-D-glucoside) [天竺葵素-3-O-(6-O-丙二酰-β-D-葡萄糖苷)]	17.9282±0.2704	21.2398±0.1752
29				Pelargonidin-3-O-galactoside[天竺葵素-3-O-半乳糖苷]	0.1132±0.0130	0.1027±0.0199
30				Pelargonidin-3-O-rutinoside-5-O-glucoside[天竺葵素-3-O-芸香糖苷-5-O-葡萄糖苷]	0.0085±0.0007	0.013 0±0.0011
31	Peonidin [芍药花素]			Peonidin-3,5-O-diglucoside[芍药花素-3,5-O-二葡萄糖苷]	0.0360±0.0008	0.0440±0.0009
32				Peonidin-3-O-(6-O-p-coumaroyl)-glucoside[芍药花素-3-(6-O-p-对香豆酰)-葡萄糖苷]	0.0377±0.0012	0.0559±0.0089
33				Peonidin-3-O-5-O-(6-O-coumaroyl)-diglucoside [芍药花素-3-O-5-O-(6-O-对香豆酰)-二葡萄糖]	0.2123±0.0189	0.2912±0.0089
34				Peonidin-3-O-glucoside[芍药花素-3-O-葡萄糖苷]	0.0058±0.0007	0.0152±0.0005
35				Peonidin-3-O-galactoside[芍药花素-3-O-半乳糖苷]	0.0415±0.0050	--
36				Peonidin-3-O-(6-O-malonyl-beta-D-glucoside) [芍药花素-3-O-(6-O-丙二酰-β-D-葡萄糖苷)]	--	0.0027±0.0002
37	Petunidin [矮牵牛素]			Petunidin-3-O-glucoside[矮牵牛素-3-O-葡萄糖苷]	0.0101±0.0006	0.0168±0.0006
38				Petunidin-3-O-(6-O-p-coumaroyl)-glucoside[矮牵牛素-3-(6-O-p-对香豆酰)-葡萄糖苷]	0.4112±0.1098	0.1144±0.0347
39				Petunidin-3-O-5-O-(6-O-coumaroyl)-diglucoside [矮牵牛素-3-O-5-O-(6-O-对香豆酰)-二葡萄糖苷]	2.1766±0.5116	0.6456±0.1385
40				Petunidin-3-O-rutinoside[矮牵牛素-3-O-芸香糖苷]	0.0131±0.0012	0.0042±0.0009
41				Petunidin-3,5-O-diglucoside[矮牵牛素-3,5-O-二葡萄糖苷]	0.0218±0.0028	--
42				Petunidin-3-O-galactoside[矮牵牛素-3-O-半乳糖苷]	0.0301±0.0068	--
43				Petunidin-3-O-(6-O-malonyl-beta-D-glucoside) [矮牵牛素-3-O-(6-O-丙二酰-β-D-葡萄糖苷)]	--	0.0200±0.0052

编号		苷元类型	化合物名称		含量/(μg/g)
编号		苷元类型	化合物名称		蓝花	白花
1		Cyanidin [矢车菊素]	Cyanidin-3,5-O-diglucoside[矢车菊素-3,5-O-二葡萄糖苷]		0.4463±0.0863	0.2100±0.0407
2			Cyanidin-3-O-glucoside[矢车菊素-3-O-葡萄糖苷]		0.0671±0.0013	0.0411±0.0021
3			Cyanidin-3-O-5-O-(6-O-coumaroyl)-diglucoside [矢车菊素-3-O-5-O-(6-O-对香豆酰)-二葡萄糖苷]		14.7591±3.364 9	7.6792±1.1506
4			Cyanidin-3,5,3'-O-triglucoside[矢车菊素-3,5,3'-O-三葡萄糖]		--	--
5			Cyanidin-3-O-arabinoside[矢车菊素-3-O-阿拉伯糖苷]		--	0.0137±0.0407
6			Cyanidin-3-O-sophoroside[矢车菊素-3-O-槐糖苷]		0.0143±0.0098	0.0143±0.0002
7		Delphinidin [飞燕草素]	Delphinidin-3-O-(6-O-malonyl)-glucoside-3'-glucoside [飞燕草素-3-O-(6-O-丙二酰基)-葡萄糖苷-3'-葡萄糖苷]		0.0522±0.0045	0.0177±0.0031
8			Delphinidin-3-O-glucoside[飞燕草素-3-O-葡萄糖苷]		0.0300±0.0029	0.0369±0.0041
9			Delphinidin-3,5-O-diglucoside[飞燕草素-3,5-O-二葡萄糖苷]		0.0215±0.0100	0.0152±0.0009
10			Delphinidin-3-O-(6-O-malonyl-beta-D-glucoside) [飞燕草素-3-O-(6-O-丙二酰-β-D-葡萄糖苷)]		0.2333±0.0255	0.0722±0.0067
11			Delphinidin-3-O-5-O-(6-O-coumaroyl)-diglucoside [飞燕草素-3-O-5-O-(6-O-对香豆酰)-二葡萄糖苷]		0.2394±0.0976	0.2460±0.0271
12			Delphinidin-3-O-galactoside[飞燕草素-3-O-半乳糖苷]		0.0519±0.0014	0.0254±0.0023
13			Delphinidin-3-O-rutinoside[飞燕草素-3-O-芸香糖苷]		0.0032±0.0006	0.0002±0.0002
14			Delphinidin-3-O-sophoroside[飞燕草素-3-O-槐糖苷]		0.1639±0.0064	0.0832±0.0012
15			Delphinidin[飞燕草素]		0.0029±0.0018	--
16		Malvidin [锦葵色素]	Malvidin-3,5-O-diglucoside[锦葵色素-3,5-O-二葡萄糖苷]		0.0561±0.0032	0.0244±0.0021
17			Malvidin-3-O-glucoside[锦葵色素-3-O-葡萄糖苷]		0.0334±0.0006	0.0174±0.0011
18			Malvidin-3-O-(6'-acetylglucoside)-5-glucoside [锦葵色素-3-O-(6'-乙酰基葡萄糖苷)-5-葡萄糖苷]		0.0067±0.0009	0.0041±0.0006
19			Malvidin-3-O-(6-O-p-coumaroyl)-glucoside[锦葵色素-3-(6-O-p-对香豆酰)-葡萄糖苷]		0.1877±0.0102	0.1131±0.0076
20			Malvidin-3-O-5-O-(6-O-coumaroyl)-diglucoside [锦葵色素-3-O-5-O-(6-O-对香豆酰)-二葡萄糖苷]		1.0690±0.0663	0.7004±0.0222
21			Malvidin-3-O-sophoroside[锦葵色素-3-O-槐糖苷]		0.0049±0.0012	0.0016±0.0006
22			Malvidin-3-O-(6-O-malonyl-beta-D-glucoside)[锦葵色素-3-O-6-O-丙二酰-β-D-葡萄糖苷]		0.0125±0.0017	--
23	Pelargonidin [天竺葵素]			Pelargonidin-3,5-O-diglucoside[天竺葵素-3,5-O-二葡萄糖苷]	0.0302±0.0080	0.0290±0.0162
24				Pelargonidin-3-O-(6-O-p-coumaroyl)-glucoside[天竺葵素-3-(6-O-p-对香豆酰)-葡萄糖苷]	1.1413±0.7978	1.6203±0.3566
25				Pelargonidin-3-O-glucoside[天竺葵素-3-O-葡萄糖苷]	2.5678±0.0492	3.1926±0.0845
26				Pelargonidin-3-O-sophoroside[天竺葵素-3-O-槐糖苷]	0.0119±0.0013	0.0191±0.0022
27				Pelargonidin-3-O-sophoroside-5-O-(malonyl)-glucoside [天竺葵素-3-O-槐糖苷-5-O-(丙二酰基)-葡萄糖苷]	0.0027±0.0006	0.0034±0.0002
28				Pelargonidin-3-O-(6-O-malonyl-beta-D-glucoside) [天竺葵素-3-O-(6-O-丙二酰-β-D-葡萄糖苷)]	17.9282±0.2704	21.2398±0.1752
29				Pelargonidin-3-O-galactoside[天竺葵素-3-O-半乳糖苷]	0.1132±0.0130	0.1027±0.0199
30				Pelargonidin-3-O-rutinoside-5-O-glucoside[天竺葵素-3-O-芸香糖苷-5-O-葡萄糖苷]	0.0085±0.0007	0.013 0±0.0011
31	Peonidin [芍药花素]			Peonidin-3,5-O-diglucoside[芍药花素-3,5-O-二葡萄糖苷]	0.0360±0.0008	0.0440±0.0009
32				Peonidin-3-O-(6-O-p-coumaroyl)-glucoside[芍药花素-3-(6-O-p-对香豆酰)-葡萄糖苷]	0.0377±0.0012	0.0559±0.0089
33				Peonidin-3-O-5-O-(6-O-coumaroyl)-diglucoside [芍药花素-3-O-5-O-(6-O-对香豆酰)-二葡萄糖]	0.2123±0.0189	0.2912±0.0089
34				Peonidin-3-O-glucoside[芍药花素-3-O-葡萄糖苷]	0.0058±0.0007	0.0152±0.0005
35				Peonidin-3-O-galactoside[芍药花素-3-O-半乳糖苷]	0.0415±0.0050	--
36				Peonidin-3-O-(6-O-malonyl-beta-D-glucoside) [芍药花素-3-O-(6-O-丙二酰-β-D-葡萄糖苷)]	--	0.0027±0.0002
37	Petunidin [矮牵牛素]			Petunidin-3-O-glucoside[矮牵牛素-3-O-葡萄糖苷]	0.0101±0.0006	0.0168±0.0006
38				Petunidin-3-O-(6-O-p-coumaroyl)-glucoside[矮牵牛素-3-(6-O-p-对香豆酰)-葡萄糖苷]	0.4112±0.1098	0.1144±0.0347
39				Petunidin-3-O-5-O-(6-O-coumaroyl)-diglucoside [矮牵牛素-3-O-5-O-(6-O-对香豆酰)-二葡萄糖苷]	2.1766±0.5116	0.6456±0.1385
40				Petunidin-3-O-rutinoside[矮牵牛素-3-O-芸香糖苷]	0.0131±0.0012	0.0042±0.0009
41				Petunidin-3,5-O-diglucoside[矮牵牛素-3,5-O-二葡萄糖苷]	0.0218±0.0028	--
42				Petunidin-3-O-galactoside[矮牵牛素-3-O-半乳糖苷]	0.0301±0.0068	--
43				Petunidin-3-O-(6-O-malonyl-beta-D-glucoside) [矮牵牛素-3-O-(6-O-丙二酰-β-D-葡萄糖苷)]	--	0.0200±0.0052