Abstract: [OThis paper aims to investigate the leaf color change under different growing stages and coloring mechanism of different Physocarpus varieties. Three Physocarpus amurensis varieties, including P. amurensis, P. amurensis‘Luteus’and P. amurensis‘Diabolo’were used as materials, we investigated the leaf characteristics of five different leaf growth stages from youth to mature. In addition, comparative analysis was conducted on characteristics in terms of leaf color change. We also examined the spatial distribution of anthocyanins glycosides in P. amurensis. The results showed that the leaf areas significantly increased as the plants grew, and P. amurensis‘Diabolo’had the biggest value of leaf area. As the leaf grew, chlorophyll a， chlorophyll b, and the ratio of chlorophyll to carotenoids showed same increasing trend. The contents of chlorophyll a and chlorophyll b of P. amurensis were extremely higher than those of the other two varieties. The leaf anthocyanins glycosides were mainly distributed in the palisade tissue, while lower in the spongy tissue and surface cells. These results above clearly explained leaf characteristics and performance of color change of the three P. amurensis varieties, and provided a theoretical basis for the scientific and reasonable application.